Naslov
Morphological analysis of renal morphology in wild-type and Tff3 knock-out mice subjected to tunicamycin-induced endoplasmic reticulum stress

Autori
Bijelić, Nikola ; Aliti, Điljferije ; Rođak, Edi ; Belovari, Tatjana ; Šešelja, Kate ; Bazina, Iva ; Baus Lončar, Mirela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Microscopy Conference MC2021 : Proceedings / - , 2021, 332-332

Skup
Microscopy Conference 2021

Mjesto i datum
Beč, Austrija, 22.08.2021. - 26.08.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
kidney ; endoplasmic reticulum stress ; Tff3 protein ; glomerulus ; tubular diameter

Sažetak
INTRODUCTION Tff3 protein is secreted by different mucosal epithelial cells and is important for protection and restoration of mucosal integrity and function. It is expressed in normal kidney tissue, but also in some renal diseases, such as oxidative stress-induced renal carcinoma and chronic kidney disease. Accumulation of unfolded proteins within the endoplasmic reticulum (ER) is one of the early events in pathological changes of different organs including kidney. Tff3 deficiency has been linked to the function of ER. OBJECTIVES The aim of this study was to asses effect of Tff3 deficiency on microscopic morphology of renal cortex and medulla in case of induced acute ER stress. MATERIALS & METHODS Five 7 weeks-old male Tff3−/−/C57BL6/NCrl mice (Tff3 knock-out mice) and five male C57BL6/NCrl (wild-type) mice were used in the study. ER stress was induced with single dose of tunicamycin (Tm) (3 µg of Tm/g of body weight), a drug that blocks the initial step of N- glycosylation, leading to accumulation of misfolded proteins in the ER. Kidneys were collected after 24 h and prepared for histological analysis. The samples were stained using PAS method. Digital images were taken (5 images of the cortex and 3 images of the medulla for each animal) and on each image representative tubules were measured using FIJI software. Arithmetic means were obtained for each sample and used for further analysis. RESULTS The analysis of renal morphometric parameters did not show significant differences in the glomerular area, tubular diameter, height of tubular epithelium and diameter of tubular lumen between wild-type mice and Tff3 knock-out mice after ER stress. CONCLUSION Tff3 deficiency does not affect tubular and glomerular morphology in applied acute ER stress model. This may point to the conclusion that Tff3 protein deficiency does not affect the cellular mechanisms during 24 h after induction of ER stress. However, model of chronic ER stress occurring in course of normal aging could provide more valuable data of Tff3 deficiency role in kidney function.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

Napomena
This research was funded by Croatian Science
Foundation grant IP-2016-06-2717. The work of
doctoral student Iva Bazina has been fully
supported by the “Young researchers career
development project – training of doctoral
students” of the Croatian Science Foundation
funded by the European Union from the European
Social Fund.
Authors wish to thank Croatian Microscopy Society
for supporting this presentation.

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