Naslov
Milk proteomics in Simmental cows with subclinical and clinical mastitis

Autori
Turk, Romana ; Rošić, Nikola ; Kuleš, Josipa ; Horvatić, Anita ; Gelemanović, Andrea ; Benić, Miroslav ; Mrljak, Vladimir ; Eckersall, David

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts, 9th International Congress Veterinary Science and Profession, Zagreb, October 9th 2021 Online / - , 2021, 106-106

Skup
9th International Congress Veterinary Science and Profession, Zagreb, October 9th 2021 Online

Mjesto i datum
Zagreb, Hrvatska, 09.10.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Mastitis, dairy cows, proteomics, pathophysiology

Sažetak
Bovine mastitis is a serious disease with great impact to dairy industry and animal welfare. This study was aimed to characterize milk proteome in naturally occurring subclinical and clinical mastitis. Milk samples from healthy Simmental cows (H, n=10) were compared to those with subclinical (SC, n=12) and clinical mastitis (CL, n=10) using tandem mass tag (TMT) proteomics. Data were analysed using Proteome Discoverer software (version 2.3., Thermo Fisher Scientific). 237 proteins were changed in abundance in both SC and CL showing different change patterns. The greatest fold changes were found in haptoglobin, serum amyloid A, beta-defensin, and serpins B3 and B1 with significant increase in both SC and CL compared to H although without significant difference between SC and CL. Proteins showed a significant increase in SC compared to H and a further significant increase in CL included alpha2-macroglobulin, immunoglobulin heavy chain, pregnancy zone protein and cathelicidin-3. There were also proteins that did not increase in SC but increased in CL including a different variant of alpha2-macroglobulin, calponin cathelicidin-1 and apolipoproteins A1 and A2. Some milk proteins decreased in response to mastitis, i.e. proteins that showed no change in SC but demonstrated a significant decrease in abundance in CL compared to healthy milk with the greatest reduction being shown by thrombospondin along with alphaS2-casein, beta-lactoglobulin and beta-casein among others. Proteins showed significant decreases in SL and further decreases in CL included an ATP-binding transporter, mucin-1 and butyrophilin. Alteration of abundance patterns of milk proteins indicate multiple protein interaction involved in complex reactions of host immune defence, acute phase response, coagulation cascade and amino acid metabolism. Identification of milk proteome is a valuable strategy for revealing potential biomarkers to be used in mastitis diagnostics, disease monitoring and therapeutic possibilities.

Izvorni jezik
Engleski

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