Naslov
Multidimensional Chromatography using Liquid Handling Platform

Autori
Dončević, Lucija ; Hozić, Amela ; Cindrić, Mario

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
19th ICS Book of Abstracts / Ašperger, Daniela ; Ukić, Šime - Zagreb : Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu, 2019, 24-24

ISBN
978-953-6470-88-4

Skup
19th International Chromatography School

Mjesto i datum
Zagreb, Hrvatska, 10.06.2019. - 11.06.2019

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
chromatography ; peptide fractions ; nanoLC-ESIqTOF

Sažetak
Multidimensional chromatography followed by electrospray ionization mass spectrometry was used to characterize two different samples, mycoplasma in human urine samples and lipids from vegerable. Automated liquid handling platform is a strong tool for fast and precise chromatographic separation which can provide 6 fractions of 96 samples simultaneously in less then 120 minutes. Although a state-of-the-art technology, lack of suitable and nonreusable commercially available cartridges limits such automated platform in scientific research. A necessity for specific chromatography separation has led us to modify commercial chromatographic cartridges. Cartridges were detached and filled with more suitable stationary phase for sample separation and method procedures with sufficient recovery rate were developed for both chromatographic sample separation. Quaternary methylammonium (QMA) was used as a strong anion-exchange stationary phase for fractionation of peptides extracted from mycoplasma. Besides, polar silica sorbent was used as a stationary phase to retain molecules in nonpolar matrices, which can effectively separate compounds with small variations in structure, such as lipids. Afterward, six fractions of each sample were analysed using LC-MS system (nanoLC-ESIqTOF) and the raw data were processed using protein database search engine. Different stationary phases can be used for the development of a new multidimensional chromatography using liquid handling platform. Commercially available cartridges can be easily reused and loaded with desirable stacionary phase. Not less important, suchs automated platforms is no longer limited and can be used for various type of samples.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kemijsko inženjerstvo

POVEZANOST RADA