Naslov
Antimicrobial susceptibility and biofilm formation ability of Staphylococcus aureus strains isolated from tibiotarsal joints of broiler breeder pullets

Autori
Lozica, Liča ; Dolenčić, Nada ; Đurašin, Ivan ; Gottstein, Željko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the 9th International Congress "Veterinary Science and Profession 2021" / Brkljača Bottegaro, N. ; Lukač, M. ; Zdolec, N. ; Vrbanac, Z. - Zagreb : Veterinarski fakultet Sveučilišta u Zagrebu, 2021, 104-104

ISBN
978-953-8006-36-4

Skup
9th International congress Veterinary science and profession

Mjesto i datum
Online, 09.10.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Staphylococcus aureus, chicken, broiler breeder, disk diffusion, antibiotic resistance, biofilm-forming ability

Sažetak
Staphylococcus aureus is a common causative agent of arthritis and synovitis in poultry. The birds with localized infection in the tibiotarsal joints and proximal flexor tendons show signs of lameness, which excludes them from reproduction and can lead to death from starvation and dehydration due to inability to walk. In this study, we investigated antimicrobial susceptibility and biofilm-forming ability of S. aureus strains isolated from Ross 308 broiler breeder pullets from two flocks. The daily mortalities were submitted to necropsy, sampled, and the swabs were bacteriologically examined. After the overnight aerobic incubation at 37ºC, the bacterial cultures were identified based on the morphology and biochemical characteristics. In total, eleven strains of S. aureus from different birds with swollen tibiotarsal joints and lesions in the gastrocnemius tendons were detected and further analysed. Antimicrobial susceptibility was determined by disk diffusion assay using amoxicillin, cephalexin, doxycycline, enrofloxacin, florfenicol, flumequine, lincomycin- spectinomycin and sulphonamides. The inhibition diameters were interpreted according to the EUCAST standards. Biofilm-forming ability was examined using the adherence quantitative assay on microtiter plates. Each strain was tested in ten replicates. The results showed all strains were resistant to sulphonamides, while seven strains (63.64%) were resistant to cephalexin. The biofilm quantification assay showed ten strains (90.91%) were moderate biofilm producers, while one strain (9.1%) was a weak biofilm producer. Due to previous problems with reovirus infection, which is also clinically manifested with arthritis, tenosynovitis and lameness, staphylococcosis was considered a secondary infection. Although the results showed low antimicrobial resistance and predominantly moderate biofilm-forming ability of the investigated strains, continuous surveillance is recommended due to high zoonotic potential of the species.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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