Naslov
Seminal plasma protein N-glycan peaks are potential predictors of semen pathology and sperm chromatin maturity in men

Autori
Marić, Tihana ; Katusić Bojanac, Ana ; Matijević, Ana ; Ceppi, Marcello ; Bruzzone, Marco ; Evgeni, Evangelini ; Petrović, Tea ; Wójcik, Iwona ; Trbojević-Akmačić, Irena ; Lauc, Gordan ; Ježek, Davor ; Fučić, Aleksandra

Izvornik
Life (2075-1729) 11 (2021), 9; 989, 16

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
male infertility ; sperm ; seminal plasma ; N-glycosylation ; DNA fragmentation ; Halosperm ; sperm chromatin maturity ; aniline blue

Sažetak
Male infertility is increasingly becoming a health and demographic problem. While it may originate from congenital or acquired diseases, it can also result from environmental exposure. Hence, the complexity of involved molecular mechanisms often requires a multiparametric approach. This study aimed to associate semen parameters with sperm DNA fragmentation, chromatin maturity and seminal plasma protein N-glycosylation. Methods: The study was conducted with 166 participants, 20–55 y old, 82 normozoospermic and 84 with pathological diagnosis. Sperm was analyzed by Halosperm assay and aniline blue staining, while seminal plasma total protein N-glycans were analyzed by ultra- high-performance liquid chromatography. Results: Sperm DNA fragmentation was significantly increased in the pathological group and was inversely correlated with sperm motility and viability. Seminal plasma total protein N-glycans were chromatographically separated in 37 individual peaks. The pattern of seminal plasma N- glycan peaks (SPGP) showed that SPGP14 significantly differs between men with normal and pathological semen parameters (p < 0.001). The multivariate analysis showed that when sperm chromatin maturity increases by 10%, SPGP17 decreases by 14% while SPGP25 increases by 25%. Conclusion: DNA integrity and seminal plasma N- glycans are associated with pathological sperm parameters. Specific N-glycans are also associated with sperm chromatin maturity and have a potential in future fertility research and clinical diagnostics.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA