Naslov
Biomarkers of effect in plasma of white stork (Ciconia ciconia) nestlings show spatial variation from Croatia

Autori
Bjedov, Dora ; Velki, Mirna ; Mikuška, Tibor ; Jurinović, Luka ; Mikuška, Alma

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10. Simpozij s međunarodnim sudjelovanjem Kopački rit Jučer, danas, sutra 2021. = 10th Symposium with international participation Kopački rit past, present, future 2021 : zbornik sažetaka = book of abstracts / Ozimec, Siniša ; Bogut, Irella ; Bogdanović, Tomislav ; Jurčević Agić, Ivančica ; Rožac, Vlatko ; Stević, Filip ; Popović, Željko ; Vereš, Marija - Osijek, 2021, 28-31

Skup
10. simpozij s međunarodnim sudjelovanjem: Kopački rit - jučer, danas, sutra = 10th Symposium with International Participation: Kopački rit - past, present, future

Mjesto i datum
Osijek, Hrvatska, 30.09.2021. - 01.10.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
blood sampling ; non-target organism ; enzymatic response ; AChE ; CES ; biomonitoring

Sažetak
Enzyme activities are extensively used biomarkers to assess the response of organisms to various chemicals from the environment. Biomarkers of effect could be used for providing crucial information and identifying harmful effects on non-target avian organisms such as white stork (Ciconia ciconia). Since white stork is an altricial species, the nestlings are suitable bioindicators by analysing biomarkers in blood. Information regarding potential exposure to harmful substances could be obtained from enzyme analysis. For this purpose, we compared biomarker response from seven locations in continental Croatia: Jakuševec (n = 8), Lonjsko polje (n = 12), Crnac polje (n = 18), Jelas polje (n = 11), Slavonski Brod − east (n = 10), Podunavlje (n = 30) and Donje Podravlje (n = 17). During breeding season 2021, 4 mL of white stork nestlings blood was sampled from brachial vein and plasma was obtained by centrifugation. Specific activities of biomarkers of effect: acetylcholinesterase (AChE) and carboxylesterase (CES) were analysed in nestlings’ plasma. Although no differences were found between the locations in AChE activity, inhibition in CES activity was recorded at Crnac polje (mean: 593.3 nmol−1min−1mL−1), Podunavlje (mean: 597.9 nmol−1min−1mL−1) and Donje Podravlje (mean: 520.0 nmol−1min−1mL−1) compared to Jakuševec (mean: 867.6 nmol−1min−1mL−1), as well as lower CES activity in Donje Podravlje compared to Jelas polje (mean: 789.2 nmol−1min−1mL−1). Exposure to chemical stressors may induce changes in enzyme activity, therefore AChE and CES are recommended for monitoring of environmental pollution for neurotoxic substances. Changes in CES activity indicate activation of detoxification mechanisms, as well as shielding AChE from inhibitory xenobiotics. Therefore, we conclude white stork nestlings may be exposed to environmental toxicants. However, a detailed multiobiomarker assessment and chemical analysis of contaminants are needed for a complete comprehension of nestling’s exposure and health status.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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