Pregled bibliografske jedinice broj: 1147197
Six-Color Confocal Immunofluorescence Microscopy with 4-Laser Lines
Six-Color Confocal Immunofluorescence Microscopy with 4-Laser Lines // Multiplexed Imaging Methods and Protocols / Eli, Zamir (ur.).
New York (NY): Springer, 2021. str. 21-30 doi:10.1007/978-1-0716-1593-5_2
CROSBI ID: 1147197 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Six-Color Confocal Immunofluorescence Microscopy
with 4-Laser Lines
Autori
Heger, Lukas ; Lühr, Jennifer J. ; Amon, Lukas ; Smith, Ana-Sunčana ; Eissing, Nathalie ; Dudziak, Diana
Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni
Knjiga
Multiplexed Imaging Methods and Protocols
Urednik/ci
Eli, Zamir
Izdavač
Springer
Grad
New York (NY)
Godina
2021
Raspon stranica
21-30
ISBN
978-1-0716-1592-8
ISSN
1064-3745
Ključne riječi
Multicolor imaging ; Fluorochrome ; Tissue Histology ; Dendritic cells ; DC
Sažetak
Confocal immunofluorescence microscopy is an advanced imaging technique routinely applied in the laboratory and clinics. Histological analyses are performed from tissue material. In general, a single fluorochrome per laser is employed, limiting simultaneous analysis to four antigens in one staining with a conventional 4-laser line microscope. Here, we describe a protocol for combining fluorochromes with the same excitation but different emission properties that allows for the analysis of six different antigens in confocal immunofluorescence microscopy with a conventional 4-laser line microscope. The proposed multiplexed method permits the identification and characterization of complex cell populations in rare tissue material.
Izvorni jezik
Engleski
Znanstvena područja
Fizika
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