Naslov
Antimikrobna rezistencija u otpadnim vodama iz sedam hrvatskih gradova
(Antimicrobial resistance in wastewaters from seven Croatian cities)

Autori
Udiković Kolić, Nikolina ; Puljko, Ana ; Milaković, Milena ; Petrić, Ines

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
56. hrvatski i 16. međunarodni simpozij agronoma : zbornik sažetaka = 56th Croatian and 16th International Symposium on Agriculture : Book of Abstracts / Rozman, Vlatka ; Antunović, Zvonko - Osijek : Fakultet agrobiotehničkih znanosti Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2021, 31-31

Skup
56. hrvatski i 16. međunarodni simpozij agronoma

Mjesto i datum
Vodice, Hrvatska, 05.09.2021. - 10.09.2021

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
antibiotic resistance ; bacteria, genes ; wastewater ; wastewater treatment plants

Sažetak
Antimicrobial resistance is an emerging concern at the global scale, threatening the effectiveness of antibiotics in treating bacterial infections. Wastewater treatment plants have been indicated as possible reservoirs and a source of dissemination of antibiotic resistant bacteria into natural watercourses, but also land through irrigation, which poses a risk to human health. The aim of this study was to analyze the abundance of bacteria and genes that show resistance to critical antibiotics in medicine, extended spectrum cephalosporins (CPS) and carbapenems, in raw and treated wastewater from wastewater treatment plants from 7 Croatian cities: Zagreb, Bjelovar, Karlovac, Čakovec, Varaždin , Vinkovci and Zadar. Culturing coliform bacteria on selective media showed that wastewater contained a significant amount of resistant coliforms (log CFU/mL = 1.43-2.26) even after the treatment process. Using quantitative PCR, CPS resistance genes (blaTEM and blaCTX-M-32) were determined in all samples and did not decrease significantly even after purification. The genes for resistance to carbapenems, blaNDM, blaKPC and blaOXA-48, were determined sporadically in treated wastewater, while the blaVIM and blaIMP genes were often detected at higher concentrations in treated than in raw wastewater. The obtained results indicate the presence of dangerous antibiotic resistance genes in the treated wastewater, so further active monitoring is needed in order to preserve the environment and human health.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Biotehnologija

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