Naslov
Proteomic Profiling In Multicentric Canine Lymphoma

Autori
Renata Barić Rafaj, Nada Kučer, Andrea Gelemanović, Ivana Rubić, Josipa Kuleš

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, ostalo

Skup
International Conferences on Science and Technology, Life Science and Technology ICONST LST 2021

Mjesto i datum
Crna Gora, 08.09.2021. - 10.09.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
dog, lymphoma, proteomic

Sažetak
Abstract: Lymphoma is the most common spontaneous malignant tumor in dogs. As a heterogeneous disease, canine lymphoma represents malignant transformation of lymphocyte subtypes at different stages of development. Different biomarkers of canine lymphoma for diagnosis, prognosis, and therapy monitoring have been of clinical interest for more than a decade. Moreover, canine lymphoma is similar to human non-Hodgkin's lymphoma and represents an excellent model as the most widely investigated tumor both in veterinary and comparative oncology. This study employed quantitative label-based proteomic and bioinformatic approaches to identify potential serum biomarkers in dogs with lymphoma. Ten dogs with multicentric lymphoma were followed in 3 time points: at the presentation to the Clinic for internal diseases, during the treatment and after the treatment. The results were compared with samples obtained from 10 healthy dogs. Results summary based on analysis of 378 proteins and four different groups of dogs: 50 proteins were significantly different between the four groups after FDR correction (all FDR < 0.05). In post-hoc tests, 152 proteins were significantly differentially abundant with adjusted P-value (FDR < 0.05) according to the group combinations (10 unique genes). Involved proteins had a role in different biological processes: response to stress, blood coagulation, regulation of biological quality, defence response, adaptive and innate immune response and acute-phase response. Differentially expressed REACTOME pathways are: complement cascade, common pathway of fibrin clot formation, signaling for integrins, innate immune system, thrombin signalling through proteinase activated receptors (PARs), platelet activation, signaling and aggregation, regulation of complement cascade and cell surface interactions at the vascular wall. Overexpressed subunit of fibrinogen - fibrinogen gamma chain isoform X2, different isforms of fibronectin and rearrangement of genes for immunoglobulin are previously connected with human cancers and non-Hodgkin's lymphoma, so they deserve further validation. Spontaneously occurring malignancies in dogs provide a tool for translational research for human oncology. Complete characterization of canine proteome in lymphoma, as well as biomarker validation with detailed explanation of the role of involved proteins becomes useful for diagnosis and treatment monitoring.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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