Naslov
Comparison of gastrointestinal stability of isothiocyanates from Tropaeolum majus L. altum using in vitro and ex vivo digestion methods

Autori
Vrca, Ivana ; Burčul, Franko ; Blažević, Ivica ; Bratanić, Andre ; Bilušić, Tea

Izvornik
Croatian journal of food science and technology (1847-3466) 13 (2021), 2; 160-166

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Tropaeolum majus L. ; benzyl isothiocyanate ; gastrointestinal stability ; in vitro digestion method ; ex vivo digestion method

Sažetak
Tropaeolum majus L. is an annual herbaceous plant and a member of the Tropaeolaceae family which belongs to the Brassicales order. It is an excellent source of flavonoids, carotenoids, phenolic acids, vitamin C, and is a plant that contains glucosinolate glucotropaeolin. The major degradation product of glucotropaeolin is benzyl isothiocyanate which is known for its various biological activities. In this study, an essential oil was isolated from the seeds of the Tropaeolum majus L. altum plant by microwave- assisted distillation and analyzed using GC-MS technique. Two compounds were identified, benzyl isothiocyanate as the major one (97.81%), and 2- phenylacetonitrile as a minor one (0.80%). Tropaeolum majus L. altum essential oil and pure benzyl isothiocyanate were then submitted to the two-phase in vitro and ex vivo digestion simulations. The analysis performed by GC-MS/MS technique showed greater stability of benzyl isothiocyanate from essential oil after in vitro (97.57%), and ex vivo (73.47%) gastric phases of the simulated digestion methods, compared to its stability after in vitro (71.17%) and ex vivo (54.90%) intestinal phases. Similar trend was shown for pure benzyl isothiocyanate.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Prehrambena tehnologija

POVEZANOST RADA