Naslov
Tropaeolum majus L. altum essential oil exhibits anticancer activity that manifests through cell morphology alterations

Autori
Vrca, Ivana ; Krce, Lucija ; Čikeš Čulić, Vedrana ; Aviani, Ivica ; Blažević, Ivica ; Bilušić, Tea

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
MC 2021 Microscopy Conference Joint Meeting of Dreiländertagung & Multinational Congress on Microscopy PROCEEDINGS / - , 2021, 333-333

Skup
MC 2021 Microscopy Conference

Mjesto i datum
Beč, Austrija, 22.08.2021. - 26.08.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Tropaeolum majus L. ; antiproliferative activity ; human bladder cancer cell line TCCSUP ; Atomic force microscopy ; optical microscopy

Sažetak
Introduction: Tropaeolum majus L. altum is a plant that belongs to Tropaeolaceae family and is known for its ornamental and medicinal properties [1]. It contains benzyl glucosinolate (glucotropaeolin) whose degradation product benzyl isothiocyanate shows various biological activities such as anticancer, antimicrobial and anti-inflammatory [2, 3, 4]. Objectives: The aim of this study was to investigate the anticancer activity of T. majus essential oil against human bladder cancer cell line TCCSUP and to determine if membrane alterations are a part of the anticancer mechanism of T. majus essential oil. Materials & methods: The essential oil of T. majus seeds was obtained by microwave-assisted distillation. Gas chromatograph equipped with mass spectrometer was used to identify volatile compounds. The half maximal inhibitory concentration (IC50) of T. majus essential oil was determined against TCCSUP cell line via the MTT assay. Atomic force and optical microscopy were used to detect morphology and membrane changes of cells treated with sub-IC50 concentration. Results: T. majus essential oil has shown anticancer activity against TCCSUP cell line due to high amount of benzyl isothiocyanate. Bright-field microscopy of sub-IC50 concentration treated cells reveals the change in cell morphology and possible membrane blebbing of some cells. Atomic force microscopy analysis suggests notable cell surface alterations/membrane bleb formation as a part of the anticancer mechanism. Conclusion: The T. majus essential oil exhibits anticancer activity against TCCSUP cancer cells by altering cell morphology after exposure with sub-IC50 concentration.

Izvorni jezik
Engleski

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