Pregled bibliografske jedinice broj: 1140525
Comparative analysis of cell wall-related proteins of different yeasts
Comparative analysis of cell wall-related proteins of different yeasts // ICY15 meets ICYGMB30 Programme & Abstracts
Beč, Austrija, 2021. str. 389-389 (poster, nije recenziran, sažetak, znanstveni)
CROSBI ID: 1140525 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Comparative analysis of cell wall-related proteins
of different yeasts
Autori
Lozančić, Mateja ; Žunar, Bojan ; Hrestak, Dora ; Lopandić, Ksenija ; Teparić, Renata ; Mrša, Vladimir
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
ICY15 meets ICYGMB30 Programme & Abstracts
/ - , 2021, 389-389
Skup
ICY15 meets ICYGMB30: The Spirit of Yeast
Mjesto i datum
Beč, Austrija, 23.08.2021. - 27.08.2021
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
Cell wall proteome, yeasts, cell wall protein profiles
Sažetak
The yeast cell wall is an extracellular structure with a complex role in preserving cell shape and integrity, and in interaction with different molecules and cells in its surrounding. Fact that 1200 Saccharomyces cerevisiae genes directly or indirectly affect cell wall formation and regulation (de Groot et al., 2001) clearly speaks of the importance of the cell wall. Here we present results from our recently published manuscript of in silico analysis comparing 187 S. cerevisiae proteins involved in cell wall biogenesis and function with cell wall proteomes of 92 different yeast species. The main goal of the research was to estimate the evolutionary conservation of proteins involved in protein glycosylation, cell wall synthesis and remodeling, and of cell wall proteins with still unknown functions. As expected, proteins involved in processes of great importance for cell wall integrity and its protective function were highly conserved. However, GPI anchored proteins involved in flocculation, aggregation, cell separation, and those with still unknown functions were not highly conserved. This analysis was coupled with analysis of proteins located in the cell wall by protein biotinylation and western blotting. Results of this analysis show distinctive protein patterns and significant differences in the overall amount of cell wall proteins for different yeast genera, and even for different species of the same genera. de Groot, P.W. et al. 2001. Comp. Funct. Genomics, 2(3), pp.124-142.
Izvorni jezik
Engleski
Znanstvena područja
Biotehnologija
POVEZANOST RADA
Projekti:
IP-2019-04-2891 - Biotehnološka primjena ugradnje heterolognih proteina u stanične stijenke kvasaca (PRODIS) (Mrša, Vladimir, HRZZ - 2019-04) ( CroRIS)
Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb
Profili:
Bojan Žunar
(autor)
Ksenija Lopandić
(autor)
Vladimir Mrša
(autor)
Dora Hrestak
(autor)
Renata Teparić
(autor)
Mateja Lozančić
(autor)