Naslov
Bacterial exposure to nickel: influence on adhesion and biofilm formation on orthodontic archwires and sensitivity to antimicrobial agents
(Bacterial exposure to nickel: influence on adhesion and biofilm formation on orthodontic archwires and sensitivity to antimicrobial agents.)

Autori
Pavlić, Andrej ; Begić, Gabrijela ; Tota, Marin ; Abram, Maja ; Špalj, Stjepan ; Gobin, Ivana

Izvornik
Materials (1996-1944) 14 (2021); 4603, 12

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
bacteria ; nickel ; biofilm ; adhesion ; orthodontic archwire ; antimicrobial resistance

Sažetak
The presence of nickel could modify bacterial behavior and susceptibility to antimicrobial agents. Adhesion and biofilm formation on orthodontic archwires can be a source of bacterial colonization and possible health hazards. Staphylococcus aureus was subjected to exposure and adaptation to various sub-inhibitory concentrations of nickel. Five strains of bacteria adapted to nickel in con-centrations of 62.5–1000 μg/mL were tested for adhesion and biofilm formation on nickel-titanium archwires. Archwires were previously incubated in artificial saliva. Bacteria were incubated with orthodontic wire with stirring for 4 h (adhesion) and 24 h (biofilm formation). The number of adherent bacteria was determined after sonication and cultivation on the Muller-Hinton agar. Disk diffusion method was performed on all bacteria to assess the differences in antimicrobial susceptibility. Bacteria adapted to lower concentrations of nickel adhered better to nickel-titanium than strains adapted to higher concentrations of nickel (p < 0.05). Biofilm formation was highest in strains adapted to 250 and 500 μg/mL of nickel (p < 0.05). The highest biofilm biomass was measured for strains adapted to 250 μg/mL, followed by those adapted to 1000 μg/mL. Bacteria adapted to lower concentrations of nickel demonstrated lower inhibition zone diameters in the disk diffusion method (p < 0.05), indicating increased antimicrobial resistance. In conclusion, bacteria adapted to 250 μg/mL of nickel ions adhered better, demonstrated higher biofilm for-mation and often had higher antimicrobial resistance than other adapted and non-adapted strains.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Dentalna medicina

Napomena
This research was funded by the Croatian Science
Foundation, grant number IP-2014-09-7500
(Immunological and regenerative implications of
corrosion of dental materials in children and
adolescents) and IP-2020-02-4027 (Environmental
factors and microbial interactions in the struc-
ture of the dental biofilm).

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