Naslov
DNA damage assessment in peripheral blood of Swiss albino mice after combined exposure to volatile anestheticsand 1 or 2 Gy radiotherapy in vivo
(DNA damage assessment in peripheral blood of Swiss albino mice after combined exposure to volatile anestheticsand 1 or 2 Gy radiotherapy in vivo)

Autori
Benković, Vesna, Borojević, Nikola ; Šikić, Dunja ; Horvat Knežević, Anica ; Milić, Mirta

Izvornik
International journal of radiation biology (0955-3002) 97 (2021), 9; 1962565, 11

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Alkaline comet assay ; Halothane ; Isoflurane ; Sevoflurane ; Swiss albino mice

Sažetak
Purpose: Patient immobilization by general volatile anesthesia (VA) may be necessary during medical radiology treatment, and its use has increased in recent years. Although ionizing radiation (IR) is a well-known genotoxic and cytotoxic agent, and VA exposure has caused a range of side effects among patients and occupationally exposed personnel, there are no studies to date comparing DNA damage effects from combined VA and single fractional IR dose exposure. Material and methods: We investigate whether there is a difference in white blood cells DNA damage response (by the alkaline comet assay) in vivo in 185 healthy Swiss albino mice divided into 37 groups, anesthetized with isoflurane/sevoflurane/halothane and exposed to 1 or 2 Gy of IR. Blood samples were taken after 0, 2, 6 and 24 h after exposure, and comet parameters were measured: tail length, tail intensity and tail moment. The cellular DNA repair index was calculated to quantify the efficiency of cells in repairing and re-joining DNA strand breaks following different treatments. Results: In combined exposures, halothane caused higher DNA damage levels that were dose- dependent ; sevoflurane damage increase did not differ significantly from the initial 1 Gy dose, and isoflurane even demonstrated a protective effect, particularly in the 2 Gy dose combined exposure. Nevertheless, none of the exposures reached control levels even after 24 h. Conclusion: Halothane appears to increase the level of radiation-induced DNA damage, while sevoflurane and isoflurane exhibited a protective effect. DNA damage may have been even greater in target organs such as liver, kidney or even the brain, and this is proposed for future study.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita, Farmacija

POVEZANOST RADA