Naslov
Clinical importance and molecular epidemiology of canine minute virus infections in dogs

Autori
Stevanović, Vladimir ; Šavorić, Juraj ; Miljan, Ana ; Kovač, Kristina ; Maurić Maljković, Maja ; Barbić, Ljubo ; Starešina, Vilim ; Martinković, Krešimir ; Hađina, Suzana ; Kovač, Snježana ; Gracin, Koraljka ; Habuš, Josipa ; Perharić, Matko ; Milas, Zoran

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts of the 11th International Congress for Veterinary Virology / - Beč : European society for veterinary virology, 2018, 112-112

Skup
11th International Congress for Veterinary Virology

Mjesto i datum
Beč, Austrija, 27.08.2018. - 30.08.2018

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Canine minute virus, epidemiology, clinical importance

Sažetak
Background Canine minute virus (CMV) is a parvovirus that affects dogs belong- ing to genus Bocaparvovirus. Even though it has been described more than fifty years ago its clinical importance and epidemiology is still unknown. In experimental infections CMV was responsible for reproductive disorders and diarrhoea in puppies while in field condi- tions diarrhoea and respiratory illness were recorded. CMV was also isolated from asymptomatic animals. The purpose of this study was to determine clinical importance of CMV infection as well as to give insights in the epidemiology of CMV infection. Methods In period from 2015 till 2017 a total of the 274 rectal swabs from dogs presented with signs of the acute gastroenteritis were collected at veterinary clinics in Zagreb. Additional rectal, nasal and vaginal swabs from 120 breeding bitches, with history of reproductive dis-orders, were sampled from 24 different breeding kennels in Croatia. As a control, rectal swabs from 75 healthy dogs were included. For all animals included in this study data, regarding signalment, medical history, course and outcome of the disease were collected as well. All collected samples were tested for presence of the CMV, using polymerase chain reaction (PCR) targeting VP2 capsid gene. For positive samples in additional PCR reactions were used to amplify most of non-structural genes. All PCR products were sequenced. Statistic description and analysis were made using Dell Statistica version 12. For phylogenetic analysis MEGA7 software was used. Results CMV infection, with prevalence of 10.22%, was significantly more prevalent (p<0.0001) in dogs with signs of gastroenteritis, than in dogs with reproduction disorders (2.5%) and in healthy population (2.67%). Statistics analysis showed significant (χ2, p=0.0013) age dis- position with no evidence of infection in animals under two months of age. Interestingly there was no more overt signs of respiratory infections in CMV infected dogs (Fisher, p=1). Sequencing and phylo- genetic analysis showed circulation of two distinct groups of strains with one group only infecting dogs between two and four months of age. There were a number of amino acid substitutions in non- structural viral proteins. Conclusion CMV seems to be an important pathogen of gastrointestinal systems of dogs in field conditions with age disposition different than the previously described. Sequencing and phylogenetic analysis showed that epidemiology of this disease is more complex than it was thought, with possible unique model of the evolution on the molecular level, which could serve as evolution model of bocavirus infections in other species.

Izvorni jezik
Hrvatski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA