Naslov
Molecular epidemiology of neuroinvasive flavivirus infections in Croatia

Autori
Bogdanić, Maja ; Savić, Vladimir ; Prpić, Jelena ; Jemeršić, Lorena ; Sabadi, Dario ; Perić, Ljiljana ; Santini, Marija ; Barbić, Ljubo ; Miklaušić- Pavić, Božana ; Tabain, Irena ; Kolarić-Sviben, Gordana ; Potočnik-Hunjadi, Tanja ; Dvorski, Elizabeta ; Butigan, Tamara ; Stevanović, Vladimir ; Zember, Sanja ; Vilibić-Čavlek, Tatjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Zbornik kratkih sadržaja / Petrović, Tamaš - Beograd : Srpsko veterinarsko društvo, 2021, 107-107

ISBN
978-86-83115-41-9

Skup
22./23. Simpozijum epizootiologa i epidemiologa

Mjesto i datum
Online, 26.04.2021. - 28.04.2021

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
neuroinvasive flaviviruses ; molecular epidemiology ; Croatia

Sažetak
(Re-)emerging flavivirus infections represent a public health problem in many parts of the world. Among neuroinvasive flaviviruses, tick-borne encephalitis virus (TBEV), West Nile virus (WNV) and Usutu virus (USUV) are most widely distributed. WNV strains are classified into several genetic lineages of which lineage 1 and 2 are the most widespread. Recent data from the European countries showed that WNV strains detected in humans mainly belong to lineage 2. USUV strains are grouped into 8 lineages: five European (1-5) and three African (1-3). Most human USUV strains belong to European USUV lineages (mainly Europe 2), however African lineages were also documented. The TBEV European subtype is most prevalent in western, northern and Eastern Europe and European parts of Russia, with Far East and Siberian subtypes co-circulating in Eastern Europe. We analyzed the molecular epidemiology of neuroinvasive flaviviruses detected in Croatia during the four consecutive transmission seasons (2017-2020). TBEV was confirmed in 43 patients (2017-2020), WNV in 61 (2017-2018) and USUV in three patients (2018). Viral RNA was detected in cerebrospinal fluid and/or urine samples using a real-time RT-PCR. Samples identified as positive using the real-time RT-PCR assays were subjected to conventional RT-PCR and Sanger sequenced. One TBEV strain detected in the urine sample of a patient with severe neuroinvasive infection (2017) belonged to TBEV European subtype. All 11 sequenced WNV strains (3 from 2017 and 8 from 2018) showed circulation of WNV lineage 2. One USUV strain from a fatal encephalitis case (2018) clustered within Europe 2 lineage. Molecular epidemiology of flaviviruses detected in Croatian patients showed a similar pattern as in other European countries.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA