Differential Serotonin Uptake Mechanisms at the Human Maternal–Fetal Interface

Baković, Petra; Kesić, Maja; Perić, Maja; Bečeheli, Ivona; Horvatiček, Marina; George, Meekha; Čičin-Šain, Lipa; Desoye, Gernot; Wadsack, Christian; Panzenboeck, Ute; Štefulj, Jasminka

doi:10.3390/ijms22157807

Pregled bibliografske jedinice broj: 1137727

Differential Serotonin Uptake Mechanisms at the Human Maternal–Fetal Interface

Baković, Petra; Kesić, Maja; Perić, Maja; Bečeheli, Ivona; Horvatiček, Marina; George, Meekha; Čičin-Šain, Lipa; Desoye, Gernot; Wadsack, Christian; Panzenboeck, Ute; Štefulj, Jasminka

Differential Serotonin Uptake Mechanisms at the Human Maternal–Fetal Interface // International journal of molecular sciences, 22 (2021), 15; 7807, 17 doi:10.3390/ijms22157807 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1137727 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Differential Serotonin Uptake Mechanisms at the Human Maternal–Fetal Interface

Autori
Baković, Petra ; Kesić, Maja ; Perić, Maja ; Bečeheli, Ivona ; Horvatiček, Marina ; George, Meekha ; Čičin-Šain, Lipa ; Desoye, Gernot ; Wadsack, Christian ; Panzenboeck, Ute ; Štefulj, Jasminka

Izvornik
International journal of molecular sciences (1422-0067) 22 (2021), 15; 7807, 17

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
5‐HT ; placenta ; cord blood ; SERT ; PMAT ; organic cation transporters ; trophoblasts ; feto‐placental endothelial cells ; platelets

Sažetak
Serotonin (5-HT) plays an extensive role during pregnancy in regulating both the placental physiology and embryonic/fetal development. The uptake of 5-HT into cells is central to the control of local concentrations of 5-HT near its molecular targets. Here, we investigated the mechanisms of 5-HT uptake into human primary placental cells and cord blood platelets, all isolated immediately after birth. Trophoblasts and cord blood platelets showed 5-HT uptake with similar Michaelis constant (Km) values (~0.6 μM), typical of the high-affinity serotonin transporter (SERT). The uptake of 5-HT into trophoblasts was efficiently inhibited by various SERT-targeting drugs. In contrast, the uptake of 5-HT into feto-placental endothelial cells was not inhibited by a SERT blocker and showed a Km value (~782 μM) in the low-affinity range. Consistent with this, SERT mRNAs were abundant in term trophoblasts but sparse in feto- placental endothelial cells, whereas the opposite was found for the low-affinity plasma membrane monoamine transporter (PMAT) mRNAs. Organic cation transporter (OCT) 1, 2, and 3 mRNAs were absent or sparse in both cell types. In summary, the results demonstrate, for the first time, the presence of functional 5-HT uptake systems in feto-placental endothelial cells and fetal platelets, cells that are in direct contact with fetal blood plasma. The data also highlight the sensitivity to various psychotropic drugs of 5-HT transport into trophoblasts facing the maternal blood. The multiple, high-, and low-affinity systems present for the cellular uptake of 5-HT underscore the importance of 5-HT homeostasis at the maternal–fetal interface.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA

Projekti:
HRZZ-IP-2018-01-6547 - Utjecaj majčinog metaboličkog stanja na serotoninski sustav posteljice i neonatusa: od metilacije DNA do funkcije proteina (PLANS) (Štefulj, Jasminka, HRZZ - 2018-01) ( CroRIS)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Jasminka Štefulj (autor)