Pregled bibliografske jedinice broj: 1137624
Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis
Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis // Glycobiology, 31 (2021), 1; 2-7 doi:10.1093/glycob/cwaa047 (međunarodna recenzija, članak, znanstveni)
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Naslov
Evaluation of different PNGase F enzymes in
immunoglobulin G and total plasma N-glycans analysis
Autori
Vilaj, Marija ; Lauc, Gordan ; Trbojević-Akmačić, Irena
Izvornik
Glycobiology (0959-6658) 31
(2021), 1;
2-7
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
N-glycans ; PNGase F ; deglycosylation ; immunoglobulin G ; plasma glycoproteins
Sažetak
Glycoproteins, proteins that are co- and posttranslationally modified by sugars (glycans), have significant roles in pathophysiology of many different diseases. One of the main steps in sample preparation for free N-glycan analysis is deglycosylation or glycan removal. The aim of this study was to compare different peptide N- glycosidase F (PNGase F) enzymes (Rapid PNGase F and two recombinant versions) for deglycosylation of total human plasma glycoproteins and different amounts of human immunoglobulin G (IgG). Deglycosylation with different PNGase F enzymes resulted in different IgG and plasma N- glycosylation hydrophilic interaction liquid chromatography ultra-performance liquid chromatography profiles. Additionally, one recombinant version of PNGase F is more efficient in deglycosylation of complex N-glycans compared with Rapid PNGase F and recombinant version of PNGase F from a different manufacturer. In terms of chromatographic peak intensities and coefficient of variation %Area values, all tested versions of PNGase F enzymes were very reproducible and on the similar level when used in optimal conditions. However, care should be taken in terms of which enzyme is used with which protocol, particularly when scaling up.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija
POVEZANOST RADA
Projekti:
EK-EFRR-KK.01.2.1.01.0003 - Nova generacija visokoprotočnih glikoservisa (Lauc, Gordan; Gornik Kljaić, Olga; Zoldoš, Vlatka, EK - Poziv na dostavu projektnih prijedloga „Povećanje razvoja novih proizvoda i usluga koji proizlaze iz aktivnosti istraživanja i razvoja“) ( CroRIS)
EK-KF-KK.01.1.1.01.0010 - Znanstveni centar izvrsnosti za personaliziranu brigu o zdravlju (ZCIPersonHealth) (Polašek, Ozren; Secenji, Aleksandar, EK ) ( CroRIS)
Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
GENOS d.o.o.
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE