Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1137624

Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis


Vilaj, Marija; Lauc, Gordan; Trbojević-Akmačić, Irena
Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis // Glycobiology, 31 (2021), 1; 2-7 doi:10.1093/glycob/cwaa047 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1137624 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis

Autori
Vilaj, Marija ; Lauc, Gordan ; Trbojević-Akmačić, Irena

Izvornik
Glycobiology (0959-6658) 31 (2021), 1; 2-7

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
N-glycans ; PNGase F ; deglycosylation ; immunoglobulin G ; plasma glycoproteins

Sažetak
Glycoproteins, proteins that are co- and posttranslationally modified by sugars (glycans), have significant roles in pathophysiology of many different diseases. One of the main steps in sample preparation for free N-glycan analysis is deglycosylation or glycan removal. The aim of this study was to compare different peptide N- glycosidase F (PNGase F) enzymes (Rapid PNGase F and two recombinant versions) for deglycosylation of total human plasma glycoproteins and different amounts of human immunoglobulin G (IgG). Deglycosylation with different PNGase F enzymes resulted in different IgG and plasma N- glycosylation hydrophilic interaction liquid chromatography ultra-performance liquid chromatography profiles. Additionally, one recombinant version of PNGase F is more efficient in deglycosylation of complex N-glycans compared with Rapid PNGase F and recombinant version of PNGase F from a different manufacturer. In terms of chromatographic peak intensities and coefficient of variation %Area values, all tested versions of PNGase F enzymes were very reproducible and on the similar level when used in optimal conditions. However, care should be taken in terms of which enzyme is used with which protocol, particularly when scaling up.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekti:
EK-EFRR-KK.01.2.1.01.0003 - Nova generacija visokoprotočnih glikoservisa (Lauc, Gordan; Gornik Kljaić, Olga; Zoldoš, Vlatka, EK - Poziv na dostavu projektnih prijedloga „Povećanje razvoja novih proizvoda i usluga koji proizlaze iz aktivnosti istraživanja i razvoja“) ( CroRIS)
EK-KF-KK.01.1.1.01.0010 - Znanstveni centar izvrsnosti za personaliziranu brigu o zdravlju (ZCIPersonHealth) (Polašek, Ozren; Secenji, Aleksandar, EK ) ( CroRIS)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
GENOS d.o.o.

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Vilaj, Marija; Lauc, Gordan; Trbojević-Akmačić, Irena
Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis // Glycobiology, 31 (2021), 1; 2-7 doi:10.1093/glycob/cwaa047 (međunarodna recenzija, članak, znanstveni)
Vilaj, M., Lauc, G. & Trbojević-Akmačić, I. (2021) Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis. Glycobiology, 31 (1), 2-7 doi:10.1093/glycob/cwaa047.
@article{article, author = {Vilaj, Marija and Lauc, Gordan and Trbojevi\'{c}-Akma\v{c}i\'{c}, Irena}, year = {2021}, pages = {2-7}, DOI = {10.1093/glycob/cwaa047}, keywords = {N-glycans, PNGase F, deglycosylation, immunoglobulin G, plasma glycoproteins}, journal = {Glycobiology}, doi = {10.1093/glycob/cwaa047}, volume = {31}, number = {1}, issn = {0959-6658}, title = {Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis}, keyword = {N-glycans, PNGase F, deglycosylation, immunoglobulin G, plasma glycoproteins} }
@article{article, author = {Vilaj, Marija and Lauc, Gordan and Trbojevi\'{c}-Akma\v{c}i\'{c}, Irena}, year = {2021}, pages = {2-7}, DOI = {10.1093/glycob/cwaa047}, keywords = {N-glycans, PNGase F, deglycosylation, immunoglobulin G, plasma glycoproteins}, journal = {Glycobiology}, doi = {10.1093/glycob/cwaa047}, volume = {31}, number = {1}, issn = {0959-6658}, title = {Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis}, keyword = {N-glycans, PNGase F, deglycosylation, immunoglobulin G, plasma glycoproteins} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font