Naslov
High-throughput rat immunoglobulin G N- glycosylation profiling revealed subclass-specific changes associated with chronic stress

Autori
Habazin, Siniša ; Mlinarević, Dražen ; Balog, Marta ; Bardak, Ana ; Gaspar, Robert ; Szűcs, Kálmán Ferenc ; Vari, Sandor G. ; Vučković, Frano ; Lauc, Gordan ; Novokmet, Mislav ; Heffer, Marija

Izvornik
Journal of Proteomics (1874-3919) 245 (2021); 104293, 9

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
N-glycans ; Glycoproteomics ; Chronic stress ; HPA axis

Sažetak
Immunoglobulin G (IgG) glycosylation corresponds well with immune system changes, so it can potentially be used as a biomarker for the consequences of chronic stress such as low-grade inflammation and enhanced immunosenescence in older animals. Here we present a high-throughput glycoproteomic workflow, including IgG enrichment, HILIC glycopeptide purification, and nano-LC-MS analysis of tryptic glycopeptides applied for the analysis of rat IgG. A cohort of 80 animals was exposed to seven stressors in a customized chronic stress protocol with blood and tissue sampling in three timepoints. Young female rats experienced an increase in agalactosylated glycoforms on IgG2a and IgG2c accompanied by a decrease in monogalactosylation. Among old females, increased galactosylation was observed in the IgG2b subclass, pointing to an anti-inflammatory activity of IgG. Additionally, IgG Fc N- glycosylation patterns in Sprague Dawley rats were analyzed, quantified, and reported for the first time. Our findings emphasize age-, sex- and subclass-dependent differences in IgG glycosylation related to chronic stress exposure, confirming the relevance of newly developed methods for further research in glycobiology of rodent immune response.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA