Naslov
Multivariate analysis as a tool for quantification of conformational transitions in DNA thin films

Autori
Serec, Kristina ; Dolanski Babić, Sanja

Izvornik
Applied sciences (Basel) (2076-3417) 11 (2021), 13; 5895, 17

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA thin films ; FTIR ; B-form quantification ; principal component analysis (PCA) ; support vector machine (SVM) ; principal component regression (PCR) ; conformational transitions ; base vibrations ; A-form ; B-form

Sažetak
The double-stranded B-form and A-form have long been considered the two most important native forms of DNA, each with its own distinct biological roles and hence the focus of many areas of study, from cellular functions to cancer diagnostics and drug treatment. Due to the heterogeneity and sensitivity of the secondary structure of DNA, there is a need for tools capable of a rapid and reliable quantification of DNA conformation in diverse environments. In this work, the second paper in the series that addresses conformational transitions in DNA thin films utilizing FTIR spectroscopy, we exploit popular chemometric methods: the principal component analysis (PCA), support vector machine (SVM) learning algorithm, and principal component regression (PCR), in order to quantify and categorize DNA conformation in thin films of different hydrated states. By complementing FTIR technique with multivariate statistical methods, we demonstrate the ability of our sample preparation and automated spectral analysis protocol to rapidly and efficiently determine conformation in DNA thin films based on the vibrational signatures in the 1800–935 cm􀀀1 range. Furthermore, we assess the impact of small hydration-related changes in FTIR spectra on automated DNA conformation detection and how to avoid discrepancies by careful sampling.

Izvorni jezik
Engleski

Znanstvena područja
Fizika

POVEZANOST RADA