Naslov
Effect of magnesium ions on the structure of DNA thin films

Autori
Serec, Kristina ; Dolanski Babić, Sanja ; Tomić, Silvia ; Podgornik, Rudolf

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
12th Christmas Biophysics Workshop / Rozman, Jan ; Ziherl, Primož - Golte, 2017, 26-26

Skup
12th Christmas Biophysics Workshop

Mjesto i datum
Golte, Slovenija, 11.12.2017. - 12.12.2017

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
DNA, konformacija DNA, interakcija Mg2+ i DNA
(DNA, DNA conformation, Interaction of the Mg+2 cations with DNA)

Sažetak
Interaction of the Mg+2 cations with DNA was previously studied by many authors as Mg ions show great affinity toward phosphate groups of DNA, thus neutralizing and stabilizing double helical structure [1-6]. However, due to great variety of experimental conditions, different authors report different affinities and preferable sites of Mg-DNA coordination, leaving particular mechanism of Mg-DNA interaction unclear. In order to address how the behavior of DNA evolves upon progressive addition of Mg2+ cations, utilizing Fourier transform infrared spectroscopy, we have investigated vibrational properties of dsDNA thin films in a broad range of added magnesium chloride salt concentration. Also, to eliminate/determine influence of intrinsic sodium and magnesium ions, we have investigated both Na-DNA and Mg-DNA thin films where Mg-DNA was obtained by extensive dialysis towards respective MgCl2 solutions. Our results demonstrate that going from the low to the high magnesium content, cation–DNA interaction changes in strength due to enhanced Debye screening, present in the both the backbone as well as the base regions [7]. Whereas lower Mg2+ salt concentrations stabilize the double-helix B conformation of dsDNA, extremely high non-physiological contents, larger than 150 mM, induce fundamental modifications of the secondary structure, which possibly indicate a transition into a more compact form.

Izvorni jezik
Engleski

Znanstvena područja
Fizika

POVEZANOST RADA