Naslov
The in vitro antimicrobial-activity approach against MDR bacteria using metal/metal-oxide nanoparticles

Autori
Kosalec, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
13th International Symposium on Pharmaceutical Sciences (ISOPS) / - , 2021

Skup
13th International Symposium on Pharmaceutical Sciences (ISOPS)

Mjesto i datum
Ankara, Turska; online, 22.06.2021. - 25.06.2021

Vrsta sudjelovanja
Plenarno

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
nanoparticles ; antimicrobial activity ; MDR bacteria

Sažetak
The use of nanoparticles (NPs) and research on antimicrobial activity is increasing. The use of NPs in catheters, on medical textiles and other surfaces in the healthcare settings is still a challenge because the non-specific nature of the antibacterial effect is different from known antimicrobial agents and biocides. An interdisciplinary approach aiming to develop the antibacterial coatings as a medical product is crucial to evaluate their antibacterial activity. The critical part of translational research is to determine the antimicrobial activities of NPs in vitro. The various in vitro approaches aim to elucidate the activity in time with different colloidal NPs in contact with bacterial cells with an endpoint such as cell viability, membrane plasticity in the cell wall and inhibition of biofilm formation. The use of traditional assays such as MIC (minimum inhibitory concentration) and MBIC (minimum biofilm inhibitory concentration) determination is compromised because NPs were inhibited by microbiological media compounds. The large surface area of NPs also aggregates in media and causes overall false negative results in antimicrobial based in vitro assays with their inhibition by proteins. On the other hand, impregnation of medical textiles with NPs coatings is challenging due to the lack of precise and accurate evaluation of antimicrobial activity of coatings. However, these drawbacks are leading to a rethinking of the in vitro antimicrobial approach to certain issues raised by the use of "classical" assays. However, some NPs, particularly those with slow oxidation, are leading to the concept of depot - as preserved antimicrobial activity in time, which represents major advantages over antimicrobials and biocides. The talk will present current in vitro approaches to antimicrobial activity of various colloidal NPs against MDR bacteria, challenges, and potential solutions. References: Rezić I, Majdak M, Ljoljić Bilić V, Pokrovac I, Martinaga L, Somogyi Škoc M, Kosalec I (2021) Development of Antibacterial Protective Coatings Active against MSSA and MRSA on Biodegradable Polymers. Polymers, 13: 659. Martinaga Pintarić L, Somogy Škoc M, Ljoljić Bilić V, Pokrovac I, Kosalec I, Rezić I (2020) Synthesis, Modification and Characterization of Antimicrobial Textile Surface Containing ZnO Nanoparticles. Polymers, 12: 1210. Fras-Zemljič L, Kosalec I, Munda M, Strnad S, Kolar M, Bračič M, Šauperl O (2015) Antimicrobial efficiency evaluation by monitoring potassium efflux for cellulose fibres functionalised by chitosan, Cellulose, 22:1933-1942.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

Napomena
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