Naslov
Exploring fruits from genus Prunus as a source of potential pharmaceutical agents – In vitro and in silico study

Autori
Popović, Boris M. ; Blagojević, Bojana ; Kucharska, Alicja Z. ; Agić, Dejan ; Magazin, Nenad ; Milović, Maja ; Serra, Ana Teresa

Vrsta, podvrsta
Radovi u časopisima, znanstveni

Izvornik
Food Chemistry (2021)

Status rada
Online first

Ključne riječi
Prunus fruits ; Polyphenols ; HT29 ; alpha-Amylase ; alpha-Glucosidase ; Dipeptidyl peptidase III

Sažetak
Prunus fruits are recognized to be rich sources of polyphenols with health promoting effect. In this work we evaluated the phenolic profile and bioactivity, namely antioxidant capacity, antiproliferative effect in HT29, and inhibition capacity of alpha-glucosidase (alpha-Gls), alpha-amylase (alpha-Amy) and human dipeptidyl peptidase III (hDPP III) activities, of traditional Prunus fruits grown in Serbia. Fifteen Prunus samples were investigated and compared: common European plum and three old plum subspecies ('vlaskaca', damson plum and white damson), purple-leaf cherry plum, red and white cherry plum, sweet cherry, sweet cherry-wild type, sour cherry, steppe cherry, mahaleb cherry, blackthorn, peach, and apricot. Principal Component Analysis highlighted steppe cherry and blackthorn as Prunus species with the highest bioactive potential. In silico analysis pointed out rutinoside derivatives of cyanidin and quercetin as the most potent inhibitors of alpha-Gls, alpha-Amy and hDPP III enzymes. Quercetin 3-O-rutinoside showed the highest binding energy to alpha-Gls (-10.6 kcal/mol).

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA