Naslov
The influence of nutrient media modification on the morphological parameters in raspberry (Rubus idaeus L.) micropropagation in the liquid and semi-solid media

Autori
Bošnjak, Dejan ; Marković, Monika ; Agić, Dejan ; Vinković, Tomislav ; Tkalec Kojić, Monika ; Ravnjak, Boris ; Stanisavljević, Aleksandar

Izvornik
Poljoprivreda (Osijek) (1330-7142) 27 (2021), 1; 22-29

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
raspberry ; micropropagation ; TIS ; medium optimization

Sažetak
Within the nursery sector, a global interest in functional fruits imposes a permanent need to improve the tissue culture technology. A modern bioreactor immersion system is currently a valid solution for the micropropagation of many cultures. At the Faculty of Agrobiotechnical Sciences Osijek (FAZOS, Croatia), a research was conducted with the aim of improving the raspberry (Rubus idaeus L., cv. HimboTop®) micropropagation protocol. The treatments implied the modifications of the MS and DKW nutrient medium (DKW – standard ; DKWm – substitution of FeEDTA with the FeEDDHA ; MSm – macroelement modification, 3 x CaCl2, MgSO4, and KH2PO4 + FeEDDHA), differently depending on the aggregate condition (semi-solid and liquid SETIS). Subsequent to 15 (SETIS) and 30 cultivation days (semi-solid medium), respectively, a morphological parameter analysis and a multiplication success evaluation were commenced. In all treatments (DKW, DKWm, and MSm), the observed parameters (shoot height, number of leaves, leaf width and length) were significantly better in an interaction with the liquid medium. Comparing the models, a liquid medium (SETIS) resulted in a significantly higher explant multiplication (4.02) (a semi-solid medium - 2.74). The best reproduction quality, implying a better plant vigor with a reduced vitrification, was obtained on the MSm medium in the SETIS. A concentration optimization of individual mesocomponents (i.e., of the macroelements) and their chemical forms (FeEDDHA) may contribute to their improved adoption and consequently to the stress suppression due to an explant transplantation. Selecting the modern technical solutions (TIS bioreactors - SETIS) and continuously improving the protocols, we may obtain the realistically sustainable models, suitable for a massive clonal reproduction.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Poljoprivreda (agronomija), Biotehnologija, Interdisciplinarne biotehničke znanosti

Napomena
This research was funded by Josip Juraj
Strossmayer University of Osijek as a part of
the UNIOS-ZUP 2018-34 Scientific Research
Project, entitled Contemporary Production of
Fruit Planting Material by in vitro Tissue
Culture (Suvremena proizvodnja voćnog sadnog
materijala in vitro kulturom tkiva).

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