Biomechanics of chromosome alignment at the spindle midplane

Risteski, Patrik; Jagrić, Mihaela; Pavin, Nenad; Tolić, Iva M.

doi:10.1016/j.cub.2021.03.082

Pregled bibliografske jedinice broj: 1130674

Biomechanics of chromosome alignment at the spindle midplane

Risteski, Patrik; Jagrić, Mihaela; Pavin, Nenad; Tolić, Iva M.

Biomechanics of chromosome alignment at the spindle midplane // Current biology, 31 (2021), 10; R574-R585 doi:10.1016/j.cub.2021.03.082 (međunarodna recenzija, pregledni rad, znanstveni)

CROSBI ID: 1130674 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Biomechanics of chromosome alignment at the spindle midplane

Autori
Risteski, Patrik ; Jagrić, Mihaela ; Pavin, Nenad ; Tolić, Iva M.

Izvornik
Current biology (0960-9822) 31 (2021), 10; R574-R585

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni

Ključne riječi
mitoza ; poravnanje kromosoma ; mikrotubuli ; diobeno vreteno ; kinezini
(mitosis ; chromosome alignment ; microtubules ; spindle ; kinesins)

Sažetak
During metaphase, chromosomes are aligned in a lineup at the equatorial plane of the spindle to ensure synchronous poleward movement of chromatids in anaphase and proper nuclear reformation at the end of mitosis. Chromosome alignment relies on microtubules, several types of motor protein and numerous other microtubule- associated and regulatory proteins. Because of the multitude of players involved, the mechanisms of chromosome alignment are still under debate. Here, we discuss the current models of alignment based on poleward pulling forces exerted onto sister kinetochores by kinetochore microtubules, which show length-dependent dynamics and undergo poleward flux, and polar ejection forces that push the chromosome arms away from the pole. We link these models with the recent ideas based on mechanical coupling between bridging and kinetochore microtubules, where sliding of bridging microtubules promotes overlap length-dependent sliding of kinetochore fibers and thus the alignment of sister kinetochores at the spindle equator. Finally, we discuss theoretical models of forces acting on chromosomes during metaphase.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija

POVEZANOST RADA

Projekti:
EK-855158 - Molekularno podrijetlo aneuploidija u zdravim i bolesnim ljudskim tkivima (ANEUPLOIDY) (Tolić, Iva; Pavin, Nenad, EK ) ( CroRIS)
EK-647077 - Nova klasa mikrotubula u vretenu koja djeluje silama na kinetohore (NewSpindleForce) (Tolić, Iva, EK ) ( CroRIS)
HRZZ-IP-2019-04-5967 - Ravnoteža sila i momenta sila u diobenom vretenu (SpindleForceBalance) (Pavin, Nenad, HRZZ - 2019-04) ( CroRIS)
EK-EFRR-KK.01.1.1.04.0057 - Inovativni protokoli mikroskopije za interdisciplinarna istraživanja u biomedicini (IPSted) (Tolić, Iva Marija, EK - KK.01.1.1.04) ( CroRIS)
--KK.01.1.1.01.0004 - Provedba vrhunskih istraživanja u sklopu Znanstvenog centra izvrsnosti za kvantne i kompleksne sustave te reprezentacije Liejevih algebri (QuantiXLie) (Buljan, Hrvoje; Pandžić, Pavle) ( CroRIS)
HRZZ-PZS-2019-02-7653 - Mehanizmi nastajanja snopova mikrotubula potrebni za sazrijevanje diobenog vretena (SpindleForm) (Tolić, Iva, HRZZ - 2019-02) ( CroRIS)