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Pregled bibliografske jedinice broj: 1130549

Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids

Malenica, Mladenka; Vukomanović, Marija; Kurtjak, Mario; Masciotti, Valentina; dal Zilio, Simone; Greco, Silvio; Lazzarino, Marco; Krušić, Vedrana; Perčić, Marko; Jelovica Badovinac, Ivana et al.
Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids // Biomedicines, 9 (2021), 6; 603, 29 doi:10.3390/biomedicines9060603 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1130549 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids

Autori
Malenica, Mladenka ; Vukomanović, Marija ; Kurtjak, Mario ; Masciotti, Valentina ; dal Zilio, Simone ; Greco, Silvio ; Lazzarino, Marco ; Krušić, Vedrana ; Perčić, Marko ; Jelovica Badovinac, Ivana ; Wechtersbach, Karmen ; Vidović, Ivona ; Baričević, Vanja ; Valić, Srećko ; Lučin, Pero ; Kojc, Nika ; Grabušić, Kristina

Izvornik
Biomedicines (2227-9059) 9 (2021), 6; 603, 29

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
extracellular vesicles ; human biofluids ; nanotechnology ; atomic force microscopy ; electron microscopy ; morphology

Sažetak
Extracellular vesicles (EVs) are nanometric membranous structures secreted from almostevery cell and present in biofluids. Because EV composition reflects the state of its parental tissue, EVs possess an enormous diagnostic/prognostic potential to reveal pathophysiological conditions.However, a prerequisite for such usage of EVs is their detailed characterisation, including visuali-sation which is mainly achieved by atomic force microscopy (AFM) and electron microscopy (EM).Here we summarise the EV preparation protocols for AFM and EM bringing out the main challengesin the imaging of EVs, both in their natural environment as biofluid constituents and in a salinesolution after EV isolation. In addition, we discuss approaches for EV imaging and identify thepotential benefits and disadvantages when different AFM and EM methods are applied, includingnumerous factors that influence the morphological characterisation, standardisation, or formationof artefacts. We also demonstrate the effects of some of these factors by using cerebrospinal fluidas an example of human biofluid with a simpler composition. Here presented comparison of ap-proaches to EV imaging should help to estimate the current state in morphology research of EVsfrom human biofluids and to identify the most efficient pathways towards the standardisation ofsample preparation and microscopy modes.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Strojarstvo, Interdisciplinarne tehničke znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA

Projekti:
HRZZ-IP-2019-04-1511 - Identifikacija cirkulirajućih biomarkera neurološkog oporavka u bolesnika s ozljedom mozga (CiMozak / CirBrain) (Grabušić, Kristina, HRZZ - 2019-04) ( CroRIS)
EK-EFRR-RC.2.2.06-0001 - Razvoj istraživačke infrastrukture na kampusu Sveučilišta u Rijeci (RISK) (Ožanić, Nevenka, EK - Operativni program Regionalna konkurentnost) ( CroRIS)
--uniri-biomed-18-279 - Uspostava protokola na Sveučilištu u Rijeci za primjenu nanotehnoloških metoda: mikroskopije atomskih sila i skenirajućeg elektronskog mikroskopa prilikom vizualizacije i karakterizacije egzosoma (Malenica Staver, Mladenka; Malenica Staver, Mladenka) ( CroRIS)
--uniri-biomed-18-5 - Izvanstanične vezikule kao klinički markeri neuroregeneracije nakon teške ozljede mozga (Grabušić, Kristina; Grabušić, Kristina) ( CroRIS)
--uniri-tehnic-18-294 - Analiza novih kompozitnih materijala i biljnih ekstrakata spektroskopskim tehnikama (Valić, Srećko) ( CroRIS)

Ustanove:
Medicinski fakultet, Rijeka,
Tehnički fakultet, Rijeka,
Institut "Ruđer Bošković", Zagreb,
Sveučilište u Rijeci - Odjel za fiziku,
Sveučilište u Rijeci,
Sveučilište u Rijeci - Odjel za biotehnologiju

Profili:

Avatar Url Marko Perčić (autor)

Avatar Url Pero Lučin (autor)

Avatar Url Mladenka Malenica (autor)

Avatar Url Kristina Grabušić (autor)

Avatar Url Srećko Valić (autor)

Avatar Url Ivana Jelovica Badovinac (autor)

Poveznice na cjeloviti tekst rada:

doi www.mdpi.com doi.org fulir.irb.hr

Citiraj ovu publikaciju:

Malenica, Mladenka; Vukomanović, Marija; Kurtjak, Mario; Masciotti, Valentina; dal Zilio, Simone; Greco, Silvio; Lazzarino, Marco; Krušić, Vedrana; Perčić, Marko; Jelovica Badovinac, Ivana et al.
Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids // Biomedicines, 9 (2021), 6; 603, 29 doi:10.3390/biomedicines9060603 (međunarodna recenzija, članak, znanstveni)
Malenica, M., Vukomanović, M., Kurtjak, M., Masciotti, V., dal Zilio, S., Greco, S., Lazzarino, M., Krušić, V., Perčić, M. & Jelovica Badovinac, I. (2021) Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids. Biomedicines, 9 (6), 603, 29 doi:10.3390/biomedicines9060603.
@article{article, author = {Malenica, Mladenka and Vukomanovi\'{c}, Marija and Kurtjak, Mario and Masciotti, Valentina and dal Zilio, Simone and Greco, Silvio and Lazzarino, Marco and Kru\v{s}i\'{c}, Vedrana and Per\v{c}i\'{c}, Marko and Jelovica Badovinac, Ivana and Wechtersbach, Karmen and Vidovi\'{c}, Ivona and Bari\v{c}evi\'{c}, Vanja and Vali\'{c}, Sre\'{c}ko and Lu\v{c}in, Pero and Kojc, Nika and Grabu\v{s}i\'{c}, Kristina}, year = {2021}, pages = {29}, DOI = {10.3390/biomedicines9060603}, chapter = {603}, keywords = {extracellular vesicles, human biofluids, nanotechnology, atomic force microscopy, electron microscopy, morphology}, journal = {Biomedicines}, doi = {10.3390/biomedicines9060603}, volume = {9}, number = {6}, issn = {2227-9059}, title = {Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids}, keyword = {extracellular vesicles, human biofluids, nanotechnology, atomic force microscopy, electron microscopy, morphology}, chapternumber = {603} }
@article{article, author = {Malenica, Mladenka and Vukomanovi\'{c}, Marija and Kurtjak, Mario and Masciotti, Valentina and dal Zilio, Simone and Greco, Silvio and Lazzarino, Marco and Kru\v{s}i\'{c}, Vedrana and Per\v{c}i\'{c}, Marko and Jelovica Badovinac, Ivana and Wechtersbach, Karmen and Vidovi\'{c}, Ivona and Bari\v{c}evi\'{c}, Vanja and Vali\'{c}, Sre\'{c}ko and Lu\v{c}in, Pero and Kojc, Nika and Grabu\v{s}i\'{c}, Kristina}, year = {2021}, pages = {29}, DOI = {10.3390/biomedicines9060603}, chapter = {603}, keywords = {extracellular vesicles, human biofluids, nanotechnology, atomic force microscopy, electron microscopy, morphology}, journal = {Biomedicines}, doi = {10.3390/biomedicines9060603}, volume = {9}, number = {6}, issn = {2227-9059}, title = {Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids}, keyword = {extracellular vesicles, human biofluids, nanotechnology, atomic force microscopy, electron microscopy, morphology}, chapternumber = {603} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus

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