Pregled bibliografske jedinice broj: 1128175
Biological Activity of Flavonoids and Rare Sesquiterpene Lactones Isolated From Centaurea ragusina L.
Biological Activity of Flavonoids and Rare Sesquiterpene Lactones Isolated From Centaurea ragusina L. // Ethnopharmacology in Central and Eastern Europe in the Context of Global Research Developments / Hohmann, Judit ; Karolina Kiss, Anna ; Rollinger, Judith Maria ; Atanasov, Atanas G. (ur.).
Lausanne: Frontiers Media, 2019. str. 20-58 doi:10.3389/fphar.2018.00972
CROSBI ID: 1128175 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Biological Activity of Flavonoids and Rare
Sesquiterpene Lactones Isolated From Centaurea
ragusina L.
Autori
Grienke, Ulrike ; Radić Brkanac, Sandra ; Vujčić, Valerija ; Urban, Ernst ; Ivanković, Siniša ; Stojković, Ranko ; Rollinger, Maria Judith ; Kralj, Juran ; Brozović, Anamaria ; Radić Stojković, Marijana
Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni
Knjiga
Ethnopharmacology in Central and Eastern Europe in the Context of Global Research Developments
Urednik/ci
Hohmann, Judit ; Karolina Kiss, Anna ; Rollinger, Judith Maria ; Atanasov, Atanas G.
Izdavač
Frontiers Media
Grad
Lausanne
Godina
2019
Raspon stranica
20-58
ISBN
978-2-88945-890-5
ISSN
1664-8714
Ključne riječi
Centaurea ; sesquiterpene lactones ; DNA ; cell viability ; toxicity ; glutathione
Sažetak
The endemic Croatian species Centaurea ragusina L., like other species from the genus Centaurea, has been traditionally used in Croatia as an antibacterial agent and for the treatment of gastrointestinal and urogenital disorders. In several chromatographic steps, three flavonoids and three sesquiterpene lactones (STLs) were isolated and identified from the most active fractions of the ethanol extract. Two STLs, one for which we created the trivial name ragusinin, and hemistepsin A are here reported for the first time as constituents of the genus Centaurea. All six compounds were screened for their effect on several tumor and one normal cell lines. Among them, ragusinin showed the best bioactivity and high specificity to affect tumor murine SCCVII, human HeLa and Caco-2 cell lines, but not the viability of normal V79 fibroblasts. Due to these characteristics the action of ragusinin was investigated in more detail. Since DNA is the primary target for many drugs with antibacterial and anticancer activity, we studied its interaction with ragusinin. Rather moderate binding affinity to DNA excluded it as the primary target of ragusinin. Due to the possibility of STL interaction with glutathione (GSH), the ubiquitous peptide that traps reactive compounds and other xenobiotics to prevent damage to vital proteins and nucleic acids, its role in deactivation of ragusinin was evaluated. Addition of the GSH precursor N- acetyl-cysteine potentiated the viability of HeLa cells, while the addition of GSH inhibitor L- buthionine sulfoximine decreased it. Moreover, pre-treatment of HeLa cells with the inhibitor of glutathione-S-transferase decreased their viability indicating the detoxifying role of GSH in ragusinin treated cells. Cell death, derived by an accumulation of cells in a G2 phase of the cell cylce, was shown to be independent of poly (ADP-ribose) polymerase and caspase-3 cleavage pointing toward an alternative cell death pathway.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija, Farmacija
POVEZANOST RADA
Projekti:
HRZZ-IP-2016-06-1036 - Određivanje ključnih molekula epitelno-mezenhimalne tranzicije kao mogućih ciljeva za terapiju raka jajnika (DEvOuT) (Brozović, Anamaria, HRZZ - 2016-06) ( CroRIS)
HRZZ-IP-2013-11-1477 - Višenamjensko očitavanje DNA/RNA sekundarne strukture molekularnim kemijskim senzorima (DNA/RNA-MolSense) (Piantanida, Ivo, HRZZ - 2013-11) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb
Profili:
Sandra Radić Brkanac
(autor)
Juran Kralj
(autor)
Siniša Ivanković
(autor)
Ranko Stojković
(autor)
Valerija Vujčić Bok
(autor)
Marijana Radić Stojković
(autor)
Anamaria Brozović
(autor)