Naslov
Analysis of RBC‐microparticles in stored whole blood bags – a promising marker to detect blood doping in sports?

Autori
Voss, Sven Christian ; Jaganjac, Morana ; Al‐ Thani, Amna Mohamed ; Grivel, Jean‐Charles ; Raynaud, Christophe Michel ; Al‐Jaber, Hind ; Al‐Menhali, Afnan Saleh ; Merenkov, Zeyed Ahmad ; Alsayrafi, Mohammed ; Latiff, Aishah ; Georgakopoulos, Costas

Izvornik
Drug Testing and Analysis (1942-7603) 9 (2017), 11-12; 1794-1798

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
autologous ; blood ; doping ; microparticles ; transfusion

Sažetak
Blood doping in sports is prohibited by the World Anti-Doping Agency (WADA). To find a possible biomarker for the detection of blood doping, we investigated the changes in blood stored in CPDA-1 blood bags of eight healthy subjects who donated one unit of blood. Aliquots were taken on days 0, 14, and 35. Platelet-free plasma was prepared and stored at -80°C until analysis on a flow cytometer dedicated for the analysis of microparticles (MPs). Changes in the number of red blood cell (RBC) -MPs were highly significant (p < 0.0001) with a mean of 219 (10^3/μL) on day 0 changing to 23 120 (10^3/μL) on day 14 and 29 310 (10^3/ μL) on day 35. We conclude that RBC-MPs seem to be a promising biomarker for doping control but confirmation by a transfusion study is necessary.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA