Naslov
Molecular organization of the Saccharomyces cerevisiae cell wall

Autori
Vuković, Renata ; Hudina-Domladovec, Marijana ; Mrša, Vladimir

Izvornik
Prehrambeno-tehnološka i biotehnološka revija (0352-9193) 32 (1994), 2-3; 99-102

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, ostalo

Ključne riječi
cell wall ; yeast cell wall ; cell wall proteins ; glucan ; mannan ; chitin

Sažetak
Saccharomyces cerevisiae cell wall is composed of a glucan network in which different mannoproteins are interspersed.2-3 % of the wall consists of chitin which plays a role in cell septation during the budding process. Interactions among wall components and the organization of these components in the cell wall are to a great deal unexplained. Recently, it has been observed that most proteins interact in vitro with glucan by hydrogen bonding at acidic pH in a rather non- specific manner. Similar experiments have, therefore, been performed with other cell wall components and it has been found that proteins interact with chitin in a similar fashion, although not as strongly as with glucan. Glucan- glucan and glucan-chitin interactions have also been observed, but no binding of either proteins, glucan, or chitin to mannan chains of mannoproteins have been detected. Cell walls of intact cells bind proteins added to the yeast suspension very poorly. Similar results have also been obtained with isolated cell walls which still retained the shape of the cell. If cells are broken in a mechanical homogenizer at acidic pH, released intracellular proteins bind to the inner side of the wall efficiently. Results suggest a layered structure of the S. cerevisiae cell wall with mannan chains forming the outer, and glucan building the inner layer.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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