Naslov
Cytogenotoxicity of Inclusion Complexes of Diazepam with 2-Hydroxypropyl-β-cyclodextrin

Autori
Hadziabdic, Jasmina ; Kopjar, Nevenka ; Želježić, Davor ; Spirtovic Halilovic Selma ; Zavrsnik, Davorka

Izvornik
Drug Research (2194-9379) 67 (2017), 11; 661-672

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
anxiolytic drugs - apoptosis - drug delivery - cytokines

Sažetak
Background Diazepam, as one of the most frequent prescribed drug from 1, 4-benzodiazepine group, has certain limitations in pharmaceutical technology due to its poor solubility in water. By forming inclusion complexes with 2-hydroxypropyl-β-cyclodextrin, diazepam's biopharmaceutical properties can be greatly improved. Aim Aim of this research was to in vitro evaluate genotoxicity of prepared novel complexes of diazepam and their influence on proliferation of human peripheral blood lymphocytes. Methods For identification of possible genotoxicity of diazepam inclusion complexes, cytokinesis-block micronucleus assay has been chosen. Evaluated concentrations of two diazepam inclusion complexes were 0.2 µg/mL, 0.5 µg/mL and 1.0 µg/mL in cell culture. For a reference, in vitro cytogenotoxicity evaluation of diazepam alone has been conducted as well. Results Neither one of the diazepam, complexed nor non-complexed, in given concentrations showed genotoxicity, induced genetic damage or loss of genetic material. Conclusions Nuclear division index values, as indicators of cytostaticity and cytotoxicity suggested that investigated inclusion diazepam complexes induced accelerated proliferation of human peripheral blood lymphocytes in vitro, therefore possibly shortening the duration and dynamics of the cell cycle.

Izvorni jezik
Engleski

Napomena
Abstracting and indexing:
Biobase Beilstein Scopus, BIOSIS, Chemical Abstracts Service (CAS), Current Contents, EMBASE Chemical Abstracts, Emerging Sources Citation Index (ESCI), Infotrieve, MEDLINE, VINITI, EBSCO, ProQuest

POVEZANOST RADA