Naslov
Lung Polar Lipid Fatty Acid Composition in the Mice After Feeding Different Lipid Supplemented Diets

Autori
Giacometti, Jasminka ; Milin, Čedomila ; Tota, Marin ; Ćuk, Mira ; Radošević-Stašić, Biserka ; Mrković, Marko ; Dobrila, Irena

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Proceedings of the 15th IFCC-FESCC European Congress of Clinical Chemistry and Laboratory Medicine / IFCC-FESCC-Euromedlab '03 - Barcelona : Monduzzi Editore S.p.A. - Medimond Inc., 2003, 409-412

Skup
European Congress of Clinical Chemistry and Laboratory Medicine

Mjesto i datum
Barcelona, Španjolska, 01.06.2003. - 05.06.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Fatty acids; Polar lipids; Lung; Olive oil diet; Corn oil diet

Sažetak
This study investigated the potential of dietary fats to modulate the fatty acid composition of mouse lung phospholipids.Changing the type and amount of lipid in the diet alters the FA composition of the lung surfactant, which may be change the physical and physiologic properties of surfactant and the alveolar surface tension. Fatty acid composition in lung polar lipids were determined in 2-3 mo old Male Balb/c mice after three weeks of feeding lipid-supplemented diets containing olive oil (5% w/w, FOO group) and corn oil (5% w/w, FCO group). Total lipids were extracted according to Folch et al., and polar lipids were separated and purified by solid-phase extraction and analysed in form of the fatty acid methyl esters by gas chromatographic analysis.Differences among groups were tested by nonparametric Kruskal-Wallis and Mann-Whitney U-test (p≤ 0.05). Similar to total lipids, phospholipids were fatty acids affected by the type of dietary oils in examined tissue.Palmitic and stearic fatty acids were major saturated fatty acids in polar lipids in the lung tissue. Feeding lipid-supplemented diet reduced C18:2/C20:4 ratio in the FOO and increased in FCO groups.The oleic acid content significantly increased in the FOO diet than in the control and FCO group, and was the major monounsaturated in the lung polar lipid tissue.Docosahexaenoic acid was the major polyunsaturated in the lung tissue sample, higher content of docosahexaenoic acid was in FOO diet than in the control and FCO group.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija

POVEZANOST RADA