Pregled bibliografske jedinice broj: 1124216
Impact of serum albumin on binding ability of dopamine and L-dopa on differently coated gold nanoparticles
Impact of serum albumin on binding ability of dopamine and L-dopa on differently coated gold nanoparticles // NanoTox 2021 Virtual Conference Book of Abstracts
Edinburgh, 2021. str. 118-118 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1124216 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Impact of serum albumin on binding ability of dopamine and L-dopa on differently coated gold nanoparticles
Autori
Kalčec, Nikolina ; Frkanec, Ruža ; Vinković Vrček, Ivana
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
NanoTox 2021 Virtual Conference Book of Abstracts
/ - Edinburgh, 2021, 118-118
Skup
10th International Conference on Nanotoxicology (NanoTox 2021)
Mjesto i datum
Edinburgh, Ujedinjeno Kraljevstvo, 20.04.2021. - 22.04.2021
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
albumin, L-dopa, dopamine, gold nanoparticles
Sažetak
Introduction Efficient and safe treatment of patients suffering Parkinson’s disease faces many challenges such as poor blood brain barrier penetration and decreased response to therapy with time. Nano-drug delivery systems may overcome these problems and can be designed to penetrate through the blood-brain barrier and interact with the central nervous system without causing any unwanted side effects. However, their efficiency largely depend on their stability in biological fluids like human plasma. This study evaluated impact of serum albumin on ability of novel gold nanoparticles (AuNPs) to be used as delivery system for dopamine or L-dopa and evaluated on their binding abilities. For this purpose, AuNPs functionalized with polyethylene glycol (PEG) and adamantane derivatives were prepared. Binding affinity of dopamine and L-dopa for these AuNPs was evaluated in the presence or absence of serum albumin using steady-state fluorescence spectroscopy. Results Differences in binding constants were observed for L-dopa/dopamine on differently functionalized AuNPs. The observed quenching mechanism was static in nature for all novel AuNPs. The presence of serum albumin interfered with L-dopa/dopamine binding on AuNPs. Conclusions Binding constants depend not only on the different functionalization of AuNPs, but also on the presence of serum protein.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Farmacija
POVEZANOST RADA
Projekti:
HRZZ-PZS-2019-02-4323 - Siguran pristup za razvoj nano-sustava za ciljanu isporuku lijekova u mozak (SENDER) (Vinković Vrček, Ivana, HRZZ ) ( CroRIS)
Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
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