Naslov
Undulations of phosphatidylcholine lipid bilayers with incorporated palmitic acid are blurred by lateral proton transfer

Autori
Brkljača, Zlatko ; Pišonić, Marina ; Crnolatac, Ivo ; Vazdar, Mario ; Bakarić, Danijela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7th European Joint Theoretical/Experimental Meeting on Membranes (EJTEMM 2021) - Book of Abstracts / Pabst, Georg ; Keller, Sandro - Graz : Universität Graz, 2021, 23-23

Skup
7th European Joint Theoretical Experimental Meeting on Membranes (EJTEMM 2021)

Mjesto i datum
Graz, Austrija, 07.04.2021. - 09.04.2021

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
lipid multibilayers ; palmitic acid ; IR spectroscopy ; DSC ; multivariate curve analysis

Sažetak
Proton transfer along biological interfaces is exceptionally difficult to unravel from both experimental and theoretical perspective [1]. In addition to rather scarce computational studies, experimental endeavours are mostly focused on the incorporation of membrane- anchored pH-sensitive dyes that detect the arrival of a migrating proton. Evidently, the establishment of a probe-free method would serve as considerable breakthrough in deepening our knowledge on lateral proton transfer. In this regard, we have examined lipid multibilayers of 1, 2-dipalmitoyl-sn-glycero-3- phosphocholine (DPPC) in the absence and the presence of small amount of palmitic acid (PA) (2 %) in buffers of pH values 4 ≤ pH ≤ 9 by FT- IR spectroscopy. Depending on the pH value, PA can be protonated (COOH) or deprotonated (COO−) and, consequently, placed at different depth of a lipid bilayer [3]. The presence of such small amount of PA should not significantly modify characteristic undulations of DPPC multibilayers surface [4], but accompanying proton transfer is expected to leave its signature. The latter are indirectly detected by FT-IR spectroscopy coupled with MCR-ALS with EFA analysis (Fig. 1) that is very sensitive and powerful tool in discerning of coupled events [5]. Experimental observations are corroborated by molecular dynamics (MD) simulations and calorimetric measurements.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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