Naslov
Dynamic Optical Displacement Spectroscopy to Quantify Biomembrane Bending Fluctuations

Autori
Monzel, Cornelia ; Schmidt, Daniel ; Seifert, Udo ; Smith, Ana-Sunčana ; Sengupta, Kheya ; Merkel, Rudolf

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Biophysical Journal, 110 / - , 2016, 487a-487a

Skup
American Biophysical Society's 60th Annual Meeting

Mjesto i datum
Los Angeles (CA), Sjedinjene Američke Države, 27.02.2016. - 02.03.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
biomembranes

Sažetak
Stochastic bending fluctuations of biomembranes are of high physiological importance, e.g., for preventing adhesion between red blood cells or for the establishment of cell substrate contacts in cell adhesion. Unfortunately, especially in nucleated cells they are notoriously difficult to quantify. To meet this challenge we developed a new technique based on Fluorescence Correlation Spectroscopy (FCS). We use comparatively high labeling of the biomembrane (∼10000 fluorophores in the focal spot) to suppress all contributions of lateral diffusion to fluctuations of the fluorescence intensity. Under these conditions all intensity variations arise from axial motion of the membrane. Best results are achieved when the focus is placed slightly above or below the membrane. The resolution, as estimated for ideal membranes is 20 nm spatial and 10 µs temporal.

Izvorni jezik
Engleski

Znanstvena područja
Fizika

POVEZANOST RADA