Pretraga

Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1116544

The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders

Milić, Mirta; Ceppi, Marcello; Bruzzone, Marco; Azqueta, Amaya; Brunborg, Gunnar; Godschalk, Roger; Koppen, Gudrun; Langie, Sabine; Møller, Peter; Teixeira, João Paulo et al.
The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders // Mutation Research/Reviews in Mutation Research, 787 (2021), 108371, 13 doi:10.1016/j.mrrev.2021.108371 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1116544 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders

Autori
Milić, Mirta ; Ceppi, Marcello ; Bruzzone, Marco ; Azqueta, Amaya ; Brunborg, Gunnar ; Godschalk, Roger ; Koppen, Gudrun ; Langie, Sabine ; Møller, Peter ; Teixeira, João Paulo ; Alija, Avdulla ; Anderson, Diana ; Andrade, Vanessa ; Andreoli, Cristina ; Asllani, Fisnik ; Bangkoglu, Ezgi Eyluel ; Barančoková, Magdalena ; Basaran, Nursen ; Boutet-Robinet, Elisa ; Buschini, Annamaria ; Cavallo, Delia ; Costa Pereira, Cristiana ; Costa, Carla ; Costa, Solange ; Da Silva, Juliana ; Del Boˊ, Cristian ; Dimitrijević Srećković, Vesna ; Djelić, Ninoslav ; Dobrzyńska, Malgorzata ; Duračková, Zdenka ; Dvořáková, Monika ; Gajski, Goran ; Galati, Serena ; García Lima, Omar ; Giovannelli, Lisa ; Goroshinskaya, Irina A. ; Grindel, Annemarie ; Gutzkow, Kristine B. ; Hernández, Alba ; Hernández, Carlos ; Holven, Kirsten B. ; Ibero-Baraibar, Idoia ; Ottestad, Inger ; Kadioglu, Ela ; Kažimirová, Alena ; Kuznetsova, Elena ; Ladeira, Carina ; Laffon, Blanca ; Lamonaca, Palma ; Lebailly, Pierre ; Louro, Henriqueta ; Mandina Cardoso, Tania ; Marcon, Francesca ; Marcos, Ricard ; Moretti, Massimo ; Moretti, Silvia ; Najafzadeh, Mojgan ; Nemeth, Zsuzsanna ; Neri, Monica ; Novotna, Bozena ; Orlow, Irene ; Paduchova, Zuzana ; Pastor, Susana ; Perdry, Hervé ; Spremo- Potparević, Biljana ; Ramadhani, Dwi ; Riso, Patrizia ; Rohr, Paula ; Rojas, Emilio ; Rossner, Pavel ; Safar, Anna ; Sardas, Semra ; Silva, Maria João ; Sirota, Nikolay ; Smolkova, Bozena ; Staruchova, Marta ; Stetina, Rudolf ; Stopper, Helga ; Surikova, Ekaterina I. ; Ulven, Stine M. ; Ursini, Cinzia Lucia ; Valdiglesias, Vanessa ; Valverde, Mahara ; Vodicka, Pavel ; Volkovova, Katarina ; Wagner, Karl-Heinz ; Živković, Lada ; Dušinská, Maria ; Collins, Andrew R. ; Bonassi, Stefano

Izvornik
Mutation Research/Reviews in Mutation Research (1383-5742) 787 (2021); 108371, 13

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Comet assay ; DNA damage ; Pooled analysis ; Human biomonitoring ; Biomarkers
(comet assay ; DNA damage ; pooled analysis ; human biomonitoring ; biomarkers)

Sažetak
The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMETproject-a COST Action launched in 2016- represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, life style and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19, 320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strandbreaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment(comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to beconsidered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Farmacija, Interdisciplinarne biotehničke znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA

Projekti:
undefined
MZOS-022-0222148-2125 - Mutageni i antimutageni u ekogenetičkim istraživanjima (Garaj-Vrhovac, Vera, MZOS ) ( CroRIS)

Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb

Profili:

Avatar Url Goran Gajski (autor)

Avatar Url Mirta Milić (autor)

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi www.sciencedirect.com

Citiraj ovu publikaciju:

Milić, Mirta; Ceppi, Marcello; Bruzzone, Marco; Azqueta, Amaya; Brunborg, Gunnar; Godschalk, Roger; Koppen, Gudrun; Langie, Sabine; Møller, Peter; Teixeira, João Paulo et al.
The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders // Mutation Research/Reviews in Mutation Research, 787 (2021), 108371, 13 doi:10.1016/j.mrrev.2021.108371 (međunarodna recenzija, članak, znanstveni)
Milić, M., Ceppi, M., Bruzzone, M., Azqueta, A., Brunborg, G., Godschalk, R., Koppen, G., Langie, S., Møller, P. & Teixeira, J. (2021) The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. Mutation Research/Reviews in Mutation Research, 787, 108371, 13 doi:10.1016/j.mrrev.2021.108371.
@article{article, author = {Mili\'{c}, Mirta and Ceppi, Marcello and Bruzzone, Marco and Azqueta, Amaya and Brunborg, Gunnar and Godschalk, Roger and Koppen, Gudrun and Langie, Sabine and M\oller, Peter and Teixeira, Jo\~{a}o Paulo and Alija, Avdulla and Anderson, Diana and Andrade, Vanessa and Andreoli, Cristina and Asllani, Fisnik and Bangkoglu, Ezgi Eyluel and Baran\v{c}okov\'{a}, Magdalena and Basaran, Nursen and Boutet-Robinet, Elisa and Buschini, Annamaria and Cavallo, Delia and Costa Pereira, Cristiana and Costa, Carla and Costa, Solange and Da Silva, Juliana and Del Boˊ, Cristian and Dimitrijevi\'{c} Sre\'{c}kovi\'{c}, Vesna and Djeli\'{c}, Ninoslav and Dobrzy\'{n}ska, Malgorzata and Dura\v{c}kov\'{a}, Zdenka and Dvo\v{r}\'{a}kov\'{a}, Monika and Gajski, Goran and Galati, Serena and Garc\'{\i}a Lima, Omar and Giovannelli, Lisa and Goroshinskaya, Irina A. and Grindel, Annemarie and Gutzkow, Kristine B. and Hern\'{a}ndez, Alba and Hern\'{a}ndez, Carlos and Holven, Kirsten B. and Ibero-Baraibar, Idoia and Ottestad, Inger and Kadioglu, Ela and Ka\v{z}imirov\'{a}, Alena and Kuznetsova, Elena and Ladeira, Carina and Laffon, Blanca and Lamonaca, Palma and Lebailly, Pierre and Louro, Henriqueta and Mandina Cardoso, Tania and Marcon, Francesca and Marcos, Ricard and Moretti, Massimo and Moretti, Silvia and Najafzadeh, Mojgan and Nemeth, Zsuzsanna and Neri, Monica and Novotna, Bozena and Orlow, Irene and Paduchova, Zuzana and Pastor, Susana and Perdry, Herv\'{e} and Spremo- Potparevi\'{c}, Biljana and Ramadhani, Dwi and Riso, Patrizia and Rohr, Paula and Rojas, Emilio and Rossner, Pavel and Safar, Anna and Sardas, Semra and Silva, Maria Jo\~{a}o and Sirota, Nikolay and Smolkova, Bozena and Staruchova, Marta and Stetina, Rudolf and Stopper, Helga and Surikova, Ekaterina I. and Ulven, Stine M. and Ursini, Cinzia Lucia and Valdiglesias, Vanessa and Valverde, Mahara and Vodicka, Pavel and Volkovova, Katarina and Wagner, Karl-Heinz and \v{Z}ivkovi\'{c}, Lada and Du\v{s}insk\'{a}, Maria and Collins, Andrew R. and Bonassi, Stefano}, year = {2021}, pages = {13}, DOI = {10.1016/j.mrrev.2021.108371}, chapter = {108371}, keywords = {Comet assay, DNA damage, Pooled analysis, Human biomonitoring, Biomarkers}, journal = {Mutation Research/Reviews in Mutation Research}, doi = {10.1016/j.mrrev.2021.108371}, volume = {787}, issn = {1383-5742}, title = {The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders}, keyword = {Comet assay, DNA damage, Pooled analysis, Human biomonitoring, Biomarkers}, chapternumber = {108371} }
@article{article, author = {Mili\'{c}, Mirta and Ceppi, Marcello and Bruzzone, Marco and Azqueta, Amaya and Brunborg, Gunnar and Godschalk, Roger and Koppen, Gudrun and Langie, Sabine and M\oller, Peter and Teixeira, Jo\~{a}o Paulo and Alija, Avdulla and Anderson, Diana and Andrade, Vanessa and Andreoli, Cristina and Asllani, Fisnik and Bangkoglu, Ezgi Eyluel and Baran\v{c}okov\'{a}, Magdalena and Basaran, Nursen and Boutet-Robinet, Elisa and Buschini, Annamaria and Cavallo, Delia and Costa Pereira, Cristiana and Costa, Carla and Costa, Solange and Da Silva, Juliana and Del Boˊ, Cristian and Dimitrijevi\'{c} Sre\'{c}kovi\'{c}, Vesna and Djeli\'{c}, Ninoslav and Dobrzy\'{n}ska, Malgorzata and Dura\v{c}kov\'{a}, Zdenka and Dvo\v{r}\'{a}kov\'{a}, Monika and Gajski, Goran and Galati, Serena and Garc\'{\i}a Lima, Omar and Giovannelli, Lisa and Goroshinskaya, Irina A. and Grindel, Annemarie and Gutzkow, Kristine B. and Hern\'{a}ndez, Alba and Hern\'{a}ndez, Carlos and Holven, Kirsten B. and Ibero-Baraibar, Idoia and Ottestad, Inger and Kadioglu, Ela and Ka\v{z}imirov\'{a}, Alena and Kuznetsova, Elena and Ladeira, Carina and Laffon, Blanca and Lamonaca, Palma and Lebailly, Pierre and Louro, Henriqueta and Mandina Cardoso, Tania and Marcon, Francesca and Marcos, Ricard and Moretti, Massimo and Moretti, Silvia and Najafzadeh, Mojgan and Nemeth, Zsuzsanna and Neri, Monica and Novotna, Bozena and Orlow, Irene and Paduchova, Zuzana and Pastor, Susana and Perdry, Herv\'{e} and Spremo- Potparevi\'{c}, Biljana and Ramadhani, Dwi and Riso, Patrizia and Rohr, Paula and Rojas, Emilio and Rossner, Pavel and Safar, Anna and Sardas, Semra and Silva, Maria Jo\~{a}o and Sirota, Nikolay and Smolkova, Bozena and Staruchova, Marta and Stetina, Rudolf and Stopper, Helga and Surikova, Ekaterina I. and Ulven, Stine M. and Ursini, Cinzia Lucia and Valdiglesias, Vanessa and Valverde, Mahara and Vodicka, Pavel and Volkovova, Katarina and Wagner, Karl-Heinz and \v{Z}ivkovi\'{c}, Lada and Du\v{s}insk\'{a}, Maria and Collins, Andrew R. and Bonassi, Stefano}, year = {2021}, pages = {13}, DOI = {10.1016/j.mrrev.2021.108371}, chapter = {108371}, keywords = {comet assay, DNA damage, pooled analysis, human biomonitoring, biomarkers}, journal = {Mutation Research/Reviews in Mutation Research}, doi = {10.1016/j.mrrev.2021.108371}, volume = {787}, issn = {1383-5742}, title = {The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders}, keyword = {comet assay, DNA damage, pooled analysis, human biomonitoring, biomarkers}, chapternumber = {108371} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

Citati:

    Altmetrijski pokazatelji:


    Podijeli:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font
    CROSBI koristi kolačiće (cookies) kako bi poboljšao funkcionalnost stranice.