Pregled bibliografske jedinice broj: 1116451
Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica
Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica // Frontiers in Cellular Neuroscience, 15 (2021), 661492, 15 doi:10.3389/fncel.2021.661492 (međunarodna recenzija, članak, znanstveni)
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Naslov
Establishment of Long-Term Primary Cortical
Neuronal Cultures From Neonatal Opossum
Monodelphis domestica
Autori
Petrović, Antonela ; Ban, Jelena ; Tomljanović, Ivana ; Pongrac, Marta ; Ivaničić, Matea ; Mikašinović, Sanja ; Mladinic, Miranda
Izvornik
Frontiers in Cellular Neuroscience (1662-5102) 15
(2021);
661492, 15
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
opossums ; cortex ; primary neuron cell culture ; radial glia cells ; astrocytes ; postnatal
Sažetak
Primary dissociated neuronal cultures have become a standard model for studying central nervous system (CNS) development. Such cultures are predominantly prepared from the hippocampus or cortex of rodents (mice and rats), while other mammals are less used. Here, we describe the establishment and extensive characterization of the primary dissociated neuronal cultures derived from the cortex of the gray South American shorttailed opossums, Monodelphis domestica. Opossums are unique in their ability to fully regenerate their CNS after an injury during their early postnatal development. Thus, we used cortex of postnatal day (P) 3–5 opossum to establish long-surviving and nearly pure neuronal cultures, as well as mixed cultures composed of radial glia cells (RGCs) in which their neurogenic and gliogenic potential was confirmed. Both types of cultures can survive for more than 1 month in vitro. We also prepared neuronal cultures from the P16–18 opossum cortex, which were composed of astrocytes and microglia, in addition to neurons. The long-surviving opossum primary dissociated neuronal cultures represent a novel mammalian in vitro platform particularly useful to study CNS development and regeneration.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
POVEZANOST RADA
Projekti:
HRZZ-IP-2016-06-7060 - Istraživanje granice između neurodegeneracije i neuroregeneracije: identifikacija ključnih molekula pomoću proteomike i funkcionalnih testova na leđnoj moždini sisavaca (DefineREgenAgeMode) (Mladinić Pejatović, Miranda, HRZZ - 2016-06) ( CroRIS)
EK-EFRR-RC.2.2.06-0001 - Razvoj istraživačke infrastrukture na kampusu Sveučilišta u Rijeci (RISK) (Ožanić, Nevenka, EK - Operativni program Regionalna konkurentnost) ( CroRIS)
Ostalo-CRP/CRO14-03 - Role of Activating Transcription Factor 3 and related molecules in the activation of spinal cord endogenous stem/progenitor cells (Mladinić Pejatović, Miranda, Ostalo - CRP - ICBEG Research Grants Programme 2014) ( CroRIS)
NadSve-Sveučilište u Rijeci-uniri-prirod-18-290 1463 - Identifikacija i kvantifikacija neuralnih matičnih stanica novom metodom optimizirane homogenizacije (Ban, Jelena, NadSve - UNIRI projekti 2018) ( CroRIS)
NadSve-Sveučilište u Rijeci-uniri-biomed-18-258 6427 - Otkrivanje ključnih molekula koje kontroliraju stanične mehanizme neuroprotekcije i neuroregeneracije posredovane proteinima toplinskog stresa nakon in vitro ozljede leđne moždine oposuma (Mladinić Pejatović, Miranda, NadSve - UNIRI projekti 2018) ( CroRIS)
Ustanove:
Medicinski fakultet, Zagreb,
Sveučilište u Rijeci - Odjel za biotehnologiju
Profili:
Marta Pongrac
(autor)
Miranda Mladinić Pejatović
(autor)
Antonela Petrović
(autor)
Jelena Ban
(autor)
Ivana Tomljanović
(autor)
Poveznice na cjeloviti tekst rada:
Pristup cjelovitom tekstu rada doi www.frontiersin.org pubmed.ncbi.nlm.nih.govCitiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus