Naslov
Composition of diatom communities on travertine barriers of the Una River (Bosnia and Herzegovina) obtained by DNA metabarcoding and morphological analysis

Autori
Kamberović, Jasmina ; Gligora Udovič, Marija ; Kahlert, Maria ; Tapolczai, Kálmán ; Lukić, Zorana ; Ahmić, Adisa ; Dedić, Anita ; Kulaš, Antonija ; Kajan, Katarina ; Hanjalić, Jasna ; Lasić, Lejla ; Kalamujic Stroil, Belma

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
ARPHA Conference Abstracts / Kahlert, Maria ; Pawlowski, Jan ; Weigand, Alex ; Bouchez ; Agnès ; Leese, Florian - : Pensoft Publishers, 2021

Skup
1st DNAQUA International Conference

Mjesto i datum
Online, 09.03.2021. - 11.03.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
diatoms ; travertine ; biodiversity ; morphological approach ; metabarcoding ; rbcL ; Una River

Sažetak
The process of travertine formation and carbonate deposition in the rivers is unique, delicate, and depends on the activity of algae and mosses. Although diatoms have been used extensively in hydrobiological studies, the comparative analysis data on diatom communities of the travertine barriers in karstic rivers are still scarce. The study aimed to detect the diatom composition on travertine barriers in the Una River, the large karstic river in Bosnia and Herzegovina. An integrated classical morphological identification approach with metabarcoding was applied on eight samples across the river length profile. Morphological analyses were performed using both light and scanning electron microscopes. Subsequent DNA metabarcoding of the chloroplastic gene 312bp rbcL was done. The DADA2 pipeline was used for the bioinformatic treatment of the demultiplexed MiSeq reads to infer Amplicon Sequence Variants (ASVs). ASVs were taxonomically assigned using the Diat.barcode v7 reference database. A total of 126 species were identified using the morphological approach, while 133 ASVs were taxonomically assigned to 58 unique taxa with the molecular approach. Diatom community structures in terms of molecular and morphological approaches were congruent with 49 shared species. Species from genera Gomphonema, Navicula and Encyonema were less assigned in molecular analysis. The most abundant taxa in the Una River are alkaliphilous, belonging to the genera Gomphonema, Nitzshia and Navicula. Although specific for their extremely good chemical status, the travertine barriers of the Una River are largely inhabited with meso-eutraphentic taxa.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

Napomena
University of Tuzla, Faculty of Natural Sciences
and Mathematics, Department of Biology, Urfeta
Vejzagića 4, B&H-75000, Tuzla, Bosnia and
Herzegovina.
University of Pannonia, Center for Natural
Science, Research Group of Limnology, Egyetem u.
10., H-8200 Veszprém, Hungary.
University of Mostar, Faculty of Science and
Education, Department of Biology, Matice hrvatske
bb, B&H-88000 Mostar, Bosnia and Herzegovina.

Society for Genetic Conservation of B&H
Endemic and Autochthonous Resources, Zmaja od
Bosne 8 Kampus,71000, Sarajevo, Bosnia and
Herzegovina.

University of Sarajevo-Institute for Genetic
Engineering and Biotechnology, Zmaja od Bosne 8
Kampus, 71000, Sarajevo, Bosnia and Herzegovina.

POVEZANOST RADA