Naslov
Protocol for enzymatic desialylation of native apo-transferrin

Autori
Friganović, Tomislav ; Borko, Valentina ; Šeba, Tino ; Kerep, Robert ; Biruš, Ivan ; Weitner, Tin

Vrsta, podvrsta
Ostale vrste radova, ostalo

Godina
2020

Ključne riječi
transferrin, desialylation, neuraminidase, enzyme

Sažetak
This protocol describes the method used for desialylation of native apo-transferrin. Desialylation is done by incubation of immobilized neuraminidase enzyme in the buffered solution of native apo-transferrin for 48 hours. After incubation, the desialylated sample is collected, washed out using a suitable buffer and concentrated by centrifugal filtration. The percentage recovery of the obtained desialylated transferrin is calculated from the absorbance at 280 nm.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA