Naslov
Chlorogenic acid ameliorates experimental colitis in mice by suppressing signaling pathways involved in inflammatory response and apoptosis

Autori
Vukelić, Iva ; Detel, Dijana ; Batičić, Lara ; Potočnjak, Iva ; Buljević, Sunčica ; Domitrović, Robert

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Alpe-Adria Rectors Conference (AARC) – International PhD Scientific Conference „Innovative technologies in biomedicine“

Mjesto i datum
Rijeka, Hrvatska, 19.09.2019. - 20.09.2019

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Chlorogenic acid ; Inflammatory bowel disease ; DSS-Induced colitis ; Apoptosis ; Oxidative stress

Sažetak
Chlorogenic acid (ChA) has multitude beneficial effects, however, underlying mechanisms by which ChA could influence the inflammation in colitis are still unknown. To investigate this, we induced colitis in mice by administration of dextran sulfate sodium (DSS) in drinking water. Oral administration of ChA significantly ameliorated clinical symptoms of DSS-induced colitis and colon shortening. ChA administration resulted in a suppression of phosphorylated extracellular signal-regulated kinases 1 and 2 (ERK1/2), c-Jun N-terminal kinases 1 and 2 (JNK1/2), Akt and signal transducer and activator of transcription 3 (STAT3) with concomitant upregulation of phosphatase and tensin homolog (PTEN) expression. ChA decreased the expression and nuclear translocation of nuclear factor-kappa B (NF-κB) p65 subunit and the expression of tumor necrosis factor-alpha (TNF-α). Induction of apoptosis and oxidative stress was attenuated by ChA through the suppression of of Bax, caspase-8, caspase-9 and heme oxygenase-1 (HO- 1) expression. The results of this study suggest that ChA could be used for the treatment of colitis by suppressing activation of pro-inflammatory and apoptotic signaling pathways.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija

POVEZANOST RADA