Pregled bibliografske jedinice broj: 1111424
Effect of electrical parameters and cholesterol concentration on giant unilamellar vesicles electroformation
Effect of electrical parameters and cholesterol concentration on giant unilamellar vesicles electroformation // Cell biochemistry and biophysics, 78 (2020), 2; 157-164 doi:10.1007/s12013-020-00910-9 (međunarodna recenzija, članak, znanstveni)
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Naslov
Effect of electrical parameters and cholesterol concentration on giant unilamellar vesicles electroformation
Autori
Boban, Zvonimir ; Puljas, Ana ; Kovač, Dubravka ; Subczynski, Witold Karol ; Raguz, Marija
Izvornik
Cell biochemistry and biophysics (1085-9195) 78
(2020), 2;
157-164
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
GUVs ; cholesterol ; phospholipids ; electroformation ; frequency ; field strength
Sažetak
Giant unilamellar vesicles (GUVs) are used extensively as models that mimic cell membranes. The cholesterol (Chol) content in the fiber cell plasma membranes of the eye lens is extremely high, exceeding the solubility threshold in the lenses of old humans. Thus, a methodological paper pertaining to preparations of model lipid bilayer membranes with high Chol content would significantly help the study of properties of these membranes. Lipid solutions containing 1-palmitoyl-2-oleoyl- glycero-3- phosphocholine (POPC) and Chol were fluorescently labeled with phospholipid analog 1, 1′-dioctadecyl-3, 3, 3′, 3′- tetramethylindocarbocyanine perchlorate (DiIC18(3)) and spin-coated to produce thin lipid films. GUVs were formed from these films using the electroformation method and the results were obtained using fluorescent microscopy. Electroformation outcomes were examined for different electrical parameters and different Chol concentrations. A wide range of field frequency–field strength (ff– fs) combinations was explored: 10–10, 000 Hz and 0.625–9.375 V/mm peak-to-peak. Optimal values for GUVs preparation were found to be 10–100 Hz and 1.25–6.25 V/mm, with largest vesicles occurring for 10 Hz and 3.75 V/mm. Chol:POPC mixing ratios (expressed as a molar ratio) ranged from 0 to 3.5. We show that increasing the Chol concentration decreases the GUVs size, but this effect can be reduced by choosing the appropriate ff–fs combination.
Izvorni jezik
Engleski
Znanstvena područja
Interdisciplinarne prirodne znanosti
POVEZANOST RADA
Projekti:
IP-2019-04-1958 - Otkrivanje organizacije i funkcije kolesterolove domene u plasma membrani fibroznih stanica leće oka uz primjenu fluorescentnih metoda (BioPhyCBD) (Raguž, Marija, HRZZ - 2019-04) ( CroRIS)
Ustanove:
Prirodoslovno-matematički fakultet, Split,
Medicinski fakultet, Split,
Sveučilište u Splitu
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus