Pregled bibliografske jedinice broj: 1110799
Biomonitoring findings for occupational lead exposure in battery and ceramic tile workers using biochemical markers, alkaline comet assay, and micronucleus test coupled with fluorescence in situ hybridisation
Biomonitoring findings for occupational lead exposure in battery and ceramic tile workers using biochemical markers, alkaline comet assay, and micronucleus test coupled with fluorescence in situ hybridisation // Arhiv za higijenu rada i toksikologiju, 71 (2020), 339-352 doi:10.2478/aiht-2020-71-3427 (međunarodna recenzija, članak, znanstveni)
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Naslov
Biomonitoring findings for occupational lead exposure in
battery and ceramic tile workers using biochemical markers,
alkaline comet assay, and micronucleus test coupled with
fluorescence in situ hybridisation
Autori
Kašuba, Vilena ; Milić, Mirta ; Želježić, Davor ; Mladinić, Marin ; Pizent, Alica ; Kljaković-Gašpić, Zorana ; Balija, Melita ; Jukić, Irena.
Izvornik
Arhiv za higijenu rada i toksikologiju (0004-1254) 71
(2020);
339-352
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
blood lead ; genetic endpoints ; genome damage ; human lymphocytes ; MN-FISH
Sažetak
Manufacture of lead-containing products has long been associated with various health risks. To get an insight into the related genotoxic risks, we conducted a biomonitoring study in 50 exposed workers and 48 matched controls using a battery of endpoints that sensitively detect the extent of genome instability in peripheral blood lymphocytes. The levels of primary DNA damage were estimated with the alkaline comet assay, while cytogenetic abnormalities were determined with the cytokinesis-block micronucleus (CBMN) cytome assay. Additionally, CBMN slides of 20 exposed and 16 control participants were subjected to fluorescence in situ hybridisation (FISH), coupled with pancentromeric probes to establish the incidence of centromere-positive micronuclei, nuclear buds, and nucleoplasmic bridges. Blood lead levels (B-Pb) were measured with atomic absorption spectrometry. To further characterise cumulative effects of occupational exposure, we measured erythrocyte protoporphyrin (EP) concentrations and delta-aminolevulinic acid dehydratase (ALAD) activity in blood. We also assessed the influence of serum folate (S-folate) and vitamin B12 (S-B12) on genome stability. Compared to controls, occupationally exposed workers demonstrated significantly higher B-Pb (298.36±162.07 vs 41.58±23.02), MN frequency (18.71±11.06 vs 8.98±7.50), centromere positive MN (C+ MN) (8.15±1.8 vs 3.69±0.47), and centromere negative MN (C- MN) (14.55±1.80 vs 4.56±0.89). Exposed women had significantly higher comet tail intensity (TI) and length (TL) than control women. Furthermore, workers showed a positive correlation between age and nuclear buds and MN, between MN and years of exposure, and between S-B12 levels and TI and ALAD activity, while a negative correlation was found between TI and B-Pb. These findings suggest that occupational settings in the manufacture of lead-containing products pose significant genotoxic risks, which calls for developing more effective work safety programmes, including periodical monitoring of B-Pb and genetic endpoints.
Izvorni jezik
Engleski
POVEZANOST RADA
Profili:
Zorana Kljaković Gašpić
(autor)
Vilena Kašuba
(autor)
Irena Jukić
(autor)
Alica Pizent
(autor)
Marin Mladinić
(autor)
Mirta Milić
(autor)
Davor Želježić
(autor)
Melita Balija
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE