Naslov
In vitro kompatibilnost prethodno zagrijanoga giomera i mikropunjenoga hibridnog kompozita

Autori
Knežević, Alena ; Želježić, Davor ; Kopjar, Nevenka ; Duarte, Jr., Sillas ; Tarle, Zrinka

Izvornik
Acta stomatologica Croatica (0001-7019) 52 (2018), 4; 286-297

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
citotoksičnost ; prethodno zagrijavanje kompozita ; temperatura ; kompozitni materijal

Sažetak
Objective: The aim of this study was to evaluate cytotoxic potencies of two light cured composite materials after heating on different temperatures and cured directly and through CAD/CAM overlay. Materials and methods: Composite materials (microfilled-hybrid Gradia Direct Posterior and Beautifil II) were heated in a Calset warming unit at three different temperatures (T1:37°C, T2:54°C, T3:68°C). A small amount of heated composite material was placed in a round mold (diameter 6mm ; 0.65mm thick), covered with Mylar sheet, pressed and polymerized with Bluephase LED unit. One group of samples were polymerized directly, and the other group through 2mm thick CAD/CAM ceramic- reinforced polymer (CRP) and CAD/CAM lithium disilicate ceramic (LDC) overlay for 20 and 40 seconds. The polymerized samples were placed immediately after curing in a lymphocyte cell culture. The viability of peripheral blood lymphocytes was evaluated using a dye exclusion technique by simultaneous staining with ethidium bromide and acridine orange. Quantitative assessments were made by determination of the percentage of viable, apoptotic and necrotic cells. The Pearson chi- square test was used for statistical analysis. Results: In case of 20 seconds polymerization, the highest number of viable cells polymerization were recorded when materials were heated at 37°C (T1), while in case of 40 seconds polymerization, the highest number of viable cells were recorded when the materials were heated at 54°C (T2). The samples polymerized through CAD/CAM overlays showed less cytotoxicity than samples polymerized directly. Conclusion: Apart from composite material composition, the cell viability was also influenced by curing time, temperature of pre-heating and polymerization pattern.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina

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