Naslov
Učinak levamisola na histološki i imunohistokemijski nalaz u tankom crijevu odbite prasadi cijepljene protiv kolibaciloze
(Effects of levamisole on histological and immunohistological parameters in the gut of weaned pigs vaccinated against colibacillosis)

Autori
Smolec, Ozren

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad

Fakultet
Veterinarski fakultet

Mjesto
Zagreb

Datum
15.06

Godina
2002

Stranica
36

Mentor
Sabočanec, Ruža

Neposredni voditelj
Božić, Frane

Ključne riječi
levamisol; odbita prasad; cijepljenje; tanka crijeva
(levamisole; weaned pigs; vaccination; small intestines)

Izvorni jezik
Hrvatski

Znanstvena područja
Veterinarska medicina

Napomena
In the present work we aimed to test the hypothesis that levamisole exerts its adjuvant activity in the vaccinated pigs by priming T lymphocytes in the gut mucosa , e.g.ileal Peyer's patch (IPP), or jejunal lamina propria (JLP).Experimeltal group of weaned pigs was primed with levamisole in immunostimulatory dose of 2, 5, mg/kg given daily , in 3 consecutive days , and controls received saline according to the same schedule.Both group were orally vaccinated with the vaccinal E.coli strain on day 0 and challenged with the virulent E.coli strain 7 days later.All pigs were sacrificed on post-challenge day 6.Expresion of CD3 antigens by IPP and LP T cells was tested using flow cytometry and immunohistochemistry.By the quantitative phenotypic analysis of isolated lymphocytes, CD3+ IPP but not LP T cells icreased in experimental vs control pigs, implying that levamisole exerts its adjuvant activity in the IPP by augmenting of cells that participate in cell-mediated immunity.The results of the quantitative phenotypic analysis of isolated cells were confirmed by immunohistochemical in situ staining.Moreover, immunohistocemical analysis of the gut mucosa revealed the presence of numerous CD3+ T cells within the villous epithelium ; however, T cells were rarely present in the LP in the primed-vaccinated pigs.Our overall data would suggest that levamisole appeared to stimulate the effector sites of the gut immune system in the vaccinated weaned pigs, serving as critical component in the establishment of protective immunity against enterotoxigenic E.coli-induced clinical disease.

POVEZANOST RADA