Naslov
Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces

Autori
Jagrić, Mihaela ; Risteski, Patrik ; Martinčić, Jelena ; Milas, Ana ; Tolić, Iva Marija

Izvornik
ELife (2050-084X) 10 (2021); E61170, 79

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
optogenetic ; mitotic spindle ; chromosome alignment

Sažetak
During metaphase, chromosome position at the spindle equator is regulated by the forces exerted by kinetochore microtubules and polar ejection forces. However, the role of forces arising from mechanical coupling of sister kinetochore fibers with bridging fibers in chromosome alignment is unknown. Here we develop an optogenetic approach for acute removal of PRC1 to partially disassemble bridging fibers and show that they promote chromosome alignment. Tracking of the plus-end protein EB3 revealed longer antiparallel overlaps of bridging microtubules upon PRC1 removal, which was accompanied by misaligned and lagging kinetochores. Kif4A/kinesin-4 and Kif18A/kinesin-8 were found within the bridging fiber and largely lost upon PRC1 removal, suggesting that these proteins regulate the overlap length of bridging microtubules. We propose that PRC1-mediated crosslinking of bridging microtubules and recruitment of kinesins to the bridging fibe promotes chromosome alignment by overlap length- dependent forces transmitted to the associated kinetochore fibers.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA