Naslov
Association between phenotype of hypovirulent Cryphonectria parasitica isolates and Cryphonectria hypovirus 1 sequence variations

Autori
Krstin, Ljiljana ; Katanić, Zorana ; Repar, Jelena ; Ježić, Marin ; Kobaš, Ana ; Lučić, Katarina ; Ćurković-Perica, Mirna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FEMS Online Conference on Microbiology - Electronic Abstract Book / - , 2020, 418-418

Skup
Federation of European Microbiological Societies Online Conference on Microbiology (FEMS 2020)

Mjesto i datum
Online, 28.10.2020. - 31.10.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
chestnut blight ; genetc variability ; growth rate ; mycovirus

Sažetak
The phytopathogenic fungus Cryphonectria parasitica is a causal agent of chestnut blight disease. The invasive pathogen was accidentally introduced from Asia to North America and Europe, where it destroyed a large number of American and European chestnut trees. This severe disease can be controlled by an RNA mycovirus, Cryphonectria hypovirus 1 (CHV1) from the family Hypoviridae. Upon infection, CHV1 causes hypovirulence, reducing sporulation, pigmentation, growth and fertility of the infected fungus. Hypovirulent isolates can promote the recovery of diseased chestnut trees previously infected with the virulent, virus-free isolates of C. parasitica. Since hypovirus infection alters the phenotypic characteristics of the fungal host, the possible association between sequence variation of the viral genome and growth of the hypovirulent C. parasitica isolates was tested. For this purpose, we analyzed partial CHV1 ORF- A sequences, determined the growth rate of hypovirulent fungal isolates and tested association between nucleotide differences in ORF-A sequences and fungal growth rates. Variability of analyzed CHV1 ORF-A sequences was high indicating high genetic diversity among analyzed CHV1 isolates. Growth rates of hypovirulent C. parasitica isolates showed considerable variation as well. An association of growth rate with an SNP within the CHV1 ORF- A sequence was implicated in the statistical analysis. However, variability of this phenotypic characteristic of hypovirulent fungal isolates seems to be only weakly associated with CHV1 ORF-A sequence variability.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Šumarstvo

POVEZANOST RADA