Naslov
Prognostic value of circulating Bcl-2 and anti- p53 antibodies in patients with breast cancer: A long term follow-up (17.5 years)

Autori
Sirotković-Skerlev, Maja ; Dedić Plavetić, Natalija ; Sedlić, Filip ; Kuna, Sanja Kusačić ; Vrbanec, Damir ; Belev, Borislav ; Pleština, Stjepko ; Kovač, Zdenko ; Kulić, Ana

Izvornik
Cancer Biomarkers (1574-0153) 22 (2020); 1-10

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Bcl-2 ; Breast cancer ; ELISA ; anti-p53 antibodies ; survival

Sažetak
Background: Apoptosis inhibition is a major tumorigenic factor. Bcl-2 dysregulation and TP53 mutation status, which may correlate with autoantibody generation, contribute to impaired apoptosis. Objective: This study aimed to investigate the prognostic value of circulating Bcl-2 and anti- p53 antibodies (p53Abs) in a 17.5- year follow- up of breast cancer patients. We also analyzed the correlations of Bcl-2 and p53Abs with various clinicopathological parameters in order to assess their impact on tumor aggressiveness. Methods: Serum Bcl-2 and p53Abs levels were analyzed by the enzyme- linked immunosorbent assay (ELISA) in 82 patients with invasive breast cancer and twenty individuals without malignancy. Results: Serum Bcl-2 and p53Abs levels in breast cancer patients were significantly higher than those in controls. Patients with high levels of Bcl-2 (cut-off 200 U/ml) had a poorer prognosis (17.5-year survival) than those with lower Bcl- 2 values. In combined analysis the subgroup of patients with elevated p53Abs (cut-off 15 U/ml) and elevated Bcl-2 (cut-offs 124 U/ml and 200 U/ml) had the worse prognosis in 17.5-year survival. In correlation analysis p53Abs and Bcl-2 were associated with unfavorable clinicopathological parameters. Conclusions: Our results suggest that breast cancer patients with high serum levels of p53Abs and Bcl-2 present an especially unfavorable group in a long follow-up.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti, Kliničke medicinske znanosti

POVEZANOST RADA