Naslov
Cytotoxic effect of ascorbic acid on osteosarcoma stem cells

Autori
Antunović, Maja ; Skube, Karlo ; Caput Mihalić, Katarina ; Pušić, Maja ; Šimić Jovičić, Marijana ; Petrović, Tadija ; Kolundžić, Robert ; Marijanović, Inga

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Cytotoxic effect of ascorbic acid on osteosarcoma stem cells / Kružić, Petar ; Caput Mihalić, Katarina ; Gottstein, Sanja ; Pavoković, Dubravko ; Kučinić, Mladen - Zagreb, 2018, 278-279

Skup
13. Hrvatski biološki kongres

Mjesto i datum
Poreč, Hrvatska, 19.09.2018. - 23.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
ascorbic acid, osteosarcoma stem cells, cytotoxicity, apoptosis

Sažetak
Osteosarcoma (OS) is a highly malignant bone tumor of children and young adults with poor survival rate. The cause lies in small population of chemotherapy-resistant cells, i.e. osteosarcoma stem cells (OS-SCs). Although ascorbic acid (AA) has long and controversial history of anti-cancer properties in vitro and in vivo, the mechanism of action has remained elusive especially in context of cancer stem cells. Thus, the aim of the study was to analyze AA as potential therapeutic for selective targeting of OS-SCs. Tumor tissue was enzymatically digested to isolate tumor cells, followed by sarcosphere assay to isolate OS-SCs, Osteosarcoma U2OS cell line, hMSCs and HEK 293 were used as control cell types. MTT assay was used to evaluate cytotoxic effect of AA (2.5 - 55 ug/ml) during 72h as well as relationship between cell number and AA concentration. While AA did not have any effect on hMSCs, U2OS and HEK 293, AA efficiently induced dose-dependent viability reduction of OS-SCs. lC50 values of AA increased with the number of seeded OS-SCs. Effect of AA on sarcosphere-forming ability was analyzed during 28 days showing successful reduction of sarcosphere formation after 6th day. AA-induced cell death type was determined by Annexin V/Pl staining showing that apoptosis was prevalent death mode with 7o% of Annexin V positive cells. Based on the obtained results, it can be concluded that AA has cytotoxic impact on OS-SCs.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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