Naslov
Levels of Neuropeptides among the Gut-brain Axis are Affected by DPPIV/CD26 Deficiency during Colon Inflammation in Mice

Autori
Baticic, Lara ; Bedoic, Edvard ; Detel, Dijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, pp prezentacija, ostalo

Izvornik
Liječnički vjesnik / Anić, Branimir - Zagreb : Hrvatski liječnički zbor, 2020, 36-36

Skup
16th International Biomedical Croatian Student Summit

Mjesto i datum
Zagreb, Hrvatska, 09.12.2020. - 12.12.2020

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Dipeptidyl-peptidase IV/CD26, Neuropeptide Y, Vasoactive intestinal peptide.

Sažetak
Neuropeptide Y (NPY) is a gut-brain peptide found in the central and enteric nervous system with controversial role in inflammatory events. Vasoactive intestinal peptide(VIP) is as well a neuropeptide secreted in brain and gut, with potential protective effect on intestinal mucosa. The hypothesis of this research was that dipeptidyl- peptidase IV/CD26 (DPP IV/CD26) plays an important role in intestinal inflammation, by influencing circulating and tissue levels of neuropeptides among the gut-brain axis. Crohn’s-like colitis model was induced in CD26 deficient and wild type mice. NPY and VIP concentrations and protein expressions as well as DPP IV/CD26 enzymatic activity have been determined at both systemic and local levels by ELISA and Western blot techniques. Our study revealed constitutionally significantly (p<0.05) higher serum VIP concentrations in conditions of CD26 deficiency, reaching their maximum values in the acute phase of colitis in serum as well as gut and brain tissue. NPY concentrations in the gut were increased in both mice strains in acute inflammation as well, with significantly (p<0.05) higher values in CD26 deficient mice. DPP IV/CD26 enzymatic activities among the gut-brain axis were also found to be altered. The results of our study indicate a causal connection between levels of NPY and VIP, inflammatory events among the gut-brain axis and the activity of DPP IV/ CD26. Therefore, DPP IV/CD26 has been confirmed to play an important neuroimmunomodulative role in colitis pathogenesis, which should further be evaluated in order to develop new potential therapeutical approaches.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA