Pregled bibliografske jedinice broj: 1098917
Transcription factor DLX6 is selectively expressed in the fetal human central amygdaloid nucleus
Transcription factor DLX6 is selectively expressed in the fetal human central amygdaloid nucleus // 11th FENS Forum of Neuroscience: book of abstracts
Berlin, Njemačka, 2018. str. 1-1 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1098917 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Transcription factor DLX6 is selectively expressed
in the fetal human central amygdaloid nucleus
Autori
Mulc, Damir ; Knezović, Vinka ; Smilović, Dinko ; Bosak, Ana ; Krsnik, Željka ; Kostović, Ivica ; Vukšić, Mario
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
11th FENS Forum of Neuroscience: book of abstracts
/ - , 2018, 1-1
Skup
11th FENS Forum of Neuroscience
Mjesto i datum
Berlin, Njemačka, 07.07.2018. - 11.07.2018
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
developmental markers ; amygdala differentiation ; gene expression
(developmental markers ; amygdala differentiation ; gene expressio)
Sažetak
Amygdala represents a key component of the limbic system involved in complex patterns of social behavior. Nevertheless, studies on its prenatal development are very scarce in humans. In our previous work we have demonstrated a human specific transient modular organization of this structure during midfetal period (Nikolic & Kostović, Anat Embryol 1986). Here we aimed to investigate possible indicators of the molecular mechanisms that underlie regional differences in maturational processes between various amygdaloid nuclei. Using publicly available gene expression database of human brain (Kang et al., Nature 2011), we selected several genes which are highly expressed prenatally in human amygdala. One of them is a transcription factor Dlx6, which is required for molecular differentiation of striatal neurons in developing mouse brain. To study DLX6 expression we employed immunohistochemistry on fixed-paraffin-embedded sections of postmortem human brains, ranging between 15th and 28th post conception weeks (PCW). The procedure for the human autopsy material was approved and controlled by the Internal Review Board of the Ethical Committee at the School of Medicine, University of Zagreb. Expression of DLX6 was visible in the amygdaloid primordium of the youngest specimen examined, in the prospective central nucleus, revealing intense nuclear staining. Using adjacent Nissl preparations, we found that DLX6+ cells correspond well with neurons displaying advanced differentiation of the nuclei (dispersed chromatin). Results indicate that DLX6 could be a part of the regulatory molecular program, presumably playing an important role in early differentiation of the central amygdaloid nucleus. Since this nucleus represents the main efferent structure, its advanced maturation supports evidence of very early establishment of amygdaloid visceromotor circuitry.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
--IP-2013-11-7379 - Histološka, MRI i analiza genske ekspresije reorganizacijskih procesa u medijalnom (limbičkom) zidu ljudskog mozga tijekom razvitka (MEDCEREB) (Vukšić, Mario) ( CroRIS)
Ustanove:
Medicinski fakultet, Zagreb
Profili:
Željka Krsnik
(autor)
Ivica Kostović
(autor)
Mario Vukšić
(autor)
Dinko Smilovic
(autor)
Vinka Knezović
(autor)
Damir Mulc
(autor)