Pregled bibliografske jedinice broj: 1095536
Differential Skewing of Circulating MR1- Restricted and γδ T Cells in Human Psoriasis Vulgaris
Differential Skewing of Circulating MR1- Restricted and γδ T Cells in Human Psoriasis Vulgaris // Frontiers in Immunology, 11 (2020), 572924, 16 doi:.org/10.3389/fimmu.2020.572924 (međunarodna recenzija, članak, znanstveni)
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Naslov
Differential Skewing of Circulating MR1-
Restricted
and γδ T Cells in Human Psoriasis Vulgaris
Autori
Plužarić, Vera ; Štefanić, Mario ; Mihalj, Martina ; Tolušić Levak, Maja ; Muršić, Ivanka ; Glavaš-Obrovac, Ljubica ; Petrek, Martin ; Balogh, Peter ; Tokić, Stana
Izvornik
Frontiers in Immunology (1664-3224) 11
(2020);
572924, 16
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
psoriasis, cytokines, gammadelta T lymphocytes, mucosal associated invariant T cells, MR1
Sažetak
Psoriasis vulgaris (PV) is a chronic, recurrent inflammatory dermatosis mediated by aberrantly activated immune cells. The role of the innate- like T cells, particularly gammadelta T (γδT) cells and MR1-restricted T lymphocytes, is incompletely explored, mainly through animal models, or by use of surrogate lineage markers, respectively. Here, we used case-control settings, multiparameter flow cytometry, 5-OP- RU-loaded MR1-tetramers, Luminex technology and targeted qRT-PCR to dissect the cellular and transcriptional landscape of γδ and MR1- restricted blood T cells in untreated PV cases (n=21, 22 matched controls). High interpersonal differences in cell composition were observed, fueling transcriptional variability at healthy baseline. A minor subset of canonical CD4+CD8+MR1-tet+TCRVα7.2+ and CD4+CD8-MR1- tet+TCRVα7.2+ T cells was the most significantly underrepresented community in male PV individuals, whereas Vδ2+ γδ T cells expressing high levels of TCR and Vδ1-δ2- γδ T cells expressing intermediate levels of TCR were selectively enriched in affected males, partly reflecting disease severity. Our findings highlight a formerly unappreciated skewing of human circulating MAIT and γδ cytomes during PV, and reveal their compositional changes in relation to sex, CMV exposure, serum cytokine content, BMI, and inflammatory burden. Complementing numerical alterations, we finally show that flow-sorted, MAIT and γδ populations exhibit divergent transcriptional changes in mild type I psoriasis, consisting of differential bulk expression for signatures of cytotoxicity/type-1 immunity (EOMES, RUNX3, IL18R), type-3 immunity (RORC, CCR6), and T cell innateness (ZBTB16).
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
POVEZANOST RADA
Projekti:
UIP-2019-04-3494 - NGS analiza transkriptoma MAIT i ydT limfocita: fenotip, funkcija i raznolikost TCR klonova u podlozi razvoja vulgarne psorijaze (NGSmyPHENOTIP) (Tokić, Stana, HRZZ - 2019-04) ( CroRIS)
Ustanove:
Klinički bolnički centar Osijek,
Medicinski fakultet, Osijek
Profili:
Martina Mihalj
(autor)
Ivanka Muršić
(autor)
Mario Štefanić
(autor)
Ljubica Glavaš Obrovac
(autor)
Maja Tolušić Levak
(autor)
Stana Tokić
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus