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Pregled bibliografske jedinice broj: 1095536

Differential Skewing of Circulating MR1- Restricted and γδ T Cells in Human Psoriasis Vulgaris


Plužarić, Vera; Štefanić, Mario; Mihalj, Martina; Tolušić Levak, Maja; Muršić, Ivanka; Glavaš-Obrovac, Ljubica; Petrek, Martin; Balogh, Peter; Tokić, Stana
Differential Skewing of Circulating MR1- Restricted and γδ T Cells in Human Psoriasis Vulgaris // Frontiers in Immunology, 11 (2020), 572924, 16 doi:.org/10.3389/fimmu.2020.572924 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1095536 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Differential Skewing of Circulating MR1- Restricted and γδ T Cells in Human Psoriasis Vulgaris

Autori
Plužarić, Vera ; Štefanić, Mario ; Mihalj, Martina ; Tolušić Levak, Maja ; Muršić, Ivanka ; Glavaš-Obrovac, Ljubica ; Petrek, Martin ; Balogh, Peter ; Tokić, Stana

Izvornik
Frontiers in Immunology (1664-3224) 11 (2020); 572924, 16

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
psoriasis, cytokines, gammadelta T lymphocytes, mucosal associated invariant T cells, MR1

Sažetak
Psoriasis vulgaris (PV) is a chronic, recurrent inflammatory dermatosis mediated by aberrantly activated immune cells. The role of the innate- like T cells, particularly gammadelta T (γδT) cells and MR1-restricted T lymphocytes, is incompletely explored, mainly through animal models, or by use of surrogate lineage markers, respectively. Here, we used case-control settings, multiparameter flow cytometry, 5-OP- RU-loaded MR1-tetramers, Luminex technology and targeted qRT-PCR to dissect the cellular and transcriptional landscape of γδ and MR1- restricted blood T cells in untreated PV cases (n=21, 22 matched controls). High interpersonal differences in cell composition were observed, fueling transcriptional variability at healthy baseline. A minor subset of canonical CD4+CD8+MR1-tet+TCRVα7.2+ and CD4+CD8-MR1- tet+TCRVα7.2+ T cells was the most significantly underrepresented community in male PV individuals, whereas Vδ2+ γδ T cells expressing high levels of TCR and Vδ1-δ2- γδ T cells expressing intermediate levels of TCR were selectively enriched in affected males, partly reflecting disease severity. Our findings highlight a formerly unappreciated skewing of human circulating MAIT and γδ cytomes during PV, and reveal their compositional changes in relation to sex, CMV exposure, serum cytokine content, BMI, and inflammatory burden. Complementing numerical alterations, we finally show that flow-sorted, MAIT and γδ populations exhibit divergent transcriptional changes in mild type I psoriasis, consisting of differential bulk expression for signatures of cytotoxicity/type-1 immunity (EOMES, RUNX3, IL18R), type-3 immunity (RORC, CCR6), and T cell innateness (ZBTB16).

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA


Projekti:
UIP-2019-04-3494 - NGS analiza transkriptoma MAIT i ydT limfocita: fenotip, funkcija i raznolikost TCR klonova u podlozi razvoja vulgarne psorijaze (NGSmyPHENOTIP) (Tokić, Stana, HRZZ - 2019-04) ( CroRIS)

Ustanove:
Klinički bolnički centar Osijek,
Medicinski fakultet, Osijek

Poveznice na cjeloviti tekst rada:

doi doi.org

Citiraj ovu publikaciju:

Plužarić, Vera; Štefanić, Mario; Mihalj, Martina; Tolušić Levak, Maja; Muršić, Ivanka; Glavaš-Obrovac, Ljubica; Petrek, Martin; Balogh, Peter; Tokić, Stana
Differential Skewing of Circulating MR1- Restricted and γδ T Cells in Human Psoriasis Vulgaris // Frontiers in Immunology, 11 (2020), 572924, 16 doi:.org/10.3389/fimmu.2020.572924 (međunarodna recenzija, članak, znanstveni)
Plužarić, V., Štefanić, M., Mihalj, M., Tolušić Levak, M., Muršić, I., Glavaš-Obrovac, L., Petrek, M., Balogh, P. & Tokić, S. (2020) Differential Skewing of Circulating MR1- Restricted and γδ T Cells in Human Psoriasis Vulgaris. Frontiers in Immunology, 11, 572924, 16 doi:.org/10.3389/fimmu.2020.572924.
@article{article, author = {Plu\v{z}ari\'{c}, Vera and \v{S}tefani\'{c}, Mario and Mihalj, Martina and Tolu\v{s}i\'{c} Levak, Maja and Mur\v{s}i\'{c}, Ivanka and Glava\v{s}-Obrovac, Ljubica and Petrek, Martin and Balogh, Peter and Toki\'{c}, Stana}, year = {2020}, pages = {16}, DOI = {doi.org/10.3389/fimmu.2020.572924}, chapter = {572924}, keywords = {psoriasis, cytokines, gammadelta T lymphocytes, mucosal associated invariant T cells, MR1}, journal = {Frontiers in Immunology}, doi = {doi.org/10.3389/fimmu.2020.572924}, volume = {11}, issn = {1664-3224}, title = {Differential Skewing of Circulating MR1- Restricted and γδ T Cells in Human Psoriasis Vulgaris}, keyword = {psoriasis, cytokines, gammadelta T lymphocytes, mucosal associated invariant T cells, MR1}, chapternumber = {572924} }
@article{article, author = {Plu\v{z}ari\'{c}, Vera and \v{S}tefani\'{c}, Mario and Mihalj, Martina and Tolu\v{s}i\'{c} Levak, Maja and Mur\v{s}i\'{c}, Ivanka and Glava\v{s}-Obrovac, Ljubica and Petrek, Martin and Balogh, Peter and Toki\'{c}, Stana}, year = {2020}, pages = {16}, DOI = {doi.org/10.3389/fimmu.2020.572924}, chapter = {572924}, keywords = {psoriasis, cytokines, gammadelta T lymphocytes, mucosal associated invariant T cells, MR1}, journal = {Frontiers in Immunology}, doi = {doi.org/10.3389/fimmu.2020.572924}, volume = {11}, issn = {1664-3224}, title = {Differential Skewing of Circulating MR1- Restricted and γδ T Cells in Human Psoriasis Vulgaris}, keyword = {psoriasis, cytokines, gammadelta T lymphocytes, mucosal associated invariant T cells, MR1}, chapternumber = {572924} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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